DCP和Glypican-3在人HCC组织中的表达及临床意义  

Expression of DCP and Glypican-3 in human hepatocellular cancer and its clinic significance

作　　者：罗红[1] 李惠[2] 

机构地区：[1]吉林省四平市中心医院病理科,吉林四平136000 [2]吉林省四平市中心医院消化内科,吉林四平136000

出　　处：《世界最新医学信息文摘》2013年第17期5-9,共5页World Latest Medicine Information Electronic Version

摘　　要：目的：研究原发性肝细胞癌（HCC）组织中DCP、Glypican-3的表达情况及其与HCC预后的关系，为其血清学研究提供组织学依据，并为HCC的预后判断提供新的指标。方法应用免疫组化方法研究DCP、Glypican-3在HCC及对照组织（n=117例）中的表达情况，以AFP作为对照，分析二种蛋白与HCC的临床、病理特征的关系，比较二者在不同组病例中的表达情况，并根据随访患者预后情况对HCC临床病理特点进行单因素及多因素分析。结果 DCP、Glypican-3及AFP在HCC中阳性表达率分别为74.32%、63.51%、51.35%，前二者在HCC组织的阳性表达高于癌旁组织，在正常肝组织中均为阴性表达，其表达的敏感度、特异度和准确度均为DCP＞Glypican-3＞AFP，三者表达无相关性（P＞0.05）。Kaplan-Meier分析及单因素分析显示：三种蛋白表达情况及术前血清AFP、肿瘤大小、分化程度、肝内转移、门脉癌栓均与术后早期复发死亡相关（P＜0.05）。COX回归分析显示肿瘤组织中的Glypican-3及DCP的表达与门脉癌栓，肿瘤大小等因素为HCC术后复发死亡的危险因素，且与HCC的复发死亡均呈正相关（P＜0.05）。结论 DCP、Glypican-3在HCC组织中有高敏感度和高特异度的表达,与目前所研究的血清学结果基本一致，并与AFP在HCC诊断及鉴别诊断的应用上互为补充，与HCC的临床病理特点及生物学行为密切相关，均可以作为HCC预后判断的独立指标。Objective To study the expression of DCP and Glypican-3 in primary hepatocellular cancer （HCC） and the relationship with prognosis of HCC, and to provide new indicators for the prognosis of HCC.Methods Immunohistochemical way was used to study the expression of DCP and Glypican-3 monoclonal antibody in HCC and control tissue（A total of 117 patients）,as AFP was used as a control. SPSS17.0 was used to analyze the relationship between the expression of the two kinds of protein and the clinical pathological features in HCC,and compare the expression of different groups,and analyze the clinical and pathological features which may affect the survival prognosis of HCC from univariate and multivariate factors. Results The positive expression rate of DCP, Glypican-3 and AFP in HCC were 74.32%, 63.51%and 51.35%respectively,and that of DCP and Glypican-3 in HCC was higher than that in adjacent tissue,and that was zero in normal liver tissue. The sensitivity,speciifcity and accuracy of the three kinds of protein expression in HCC were all DCP 〉 Glypican-3 〉 AFP,and no correlation of each other（P〉 0.05）.It was showed that three kinds of protein and preoperative serum AFP, tumor size, differentiation, intrahepatic metastasis, portal vein thrombosis were associated with early postoperative recurrence and death by Kaplan-Meier and univariate analysis,and the expression of Glypican-3 and DCP in tumor tissue and portal vein thrombosis, tumor size of HCC were risk factors ,and were positively correlated of recurrence and death of HCC by COX regression analysis（P〈0.05）.Conclusion DCP and Glypican-3 were studied that whose expression of in HCC tissue show high sensitivity and high speciifcity,,which consistent approximately with currently serological studies and are complementary with AFP in diagnosis and differential diagnosis of HCC, and are closely related to clinical-pathological features and biological behavior of HCC, can be used as an independent predictor of HCC prognosis.

关 键 词：原发性HCC DCP GLYPICAN-3 免疫组化 诊断 预后 

分 类 号：R445[医药卫生—影像医学与核医学]