吉林省2011年<5岁婴幼儿诺如病毒感染流行病学特征及基因型研究  

Epidemiological Characteristics and Genotype of Norovirus in Infants under 5 Years Old in Jilin Province in 2011

作　　者：李响[1] 杨显达[1] 许爽[1] 吴东林[1] 孙利炜[2] 卢欣荣[1] 沈博[1] 柳鸿敏[1] 李静[1] 

机构地区：[1]吉林省疾病预防控制中心,长春130062 [2]长春市儿童医院,吉林长春130061

出　　处：《中国疫苗和免疫》2013年第5期431-434,共4页Chinese Journal of Vaccines and Immunization

基　　金：国家科学技术艾滋病和病毒性肝炎等重大传染病防治重大专项传染病监测技术平台项目;辽宁及周边省市传染病病原谱流行规律研究课题;吉林省传染病病原谱流行规律研究子课题(编号:2012ZX10004209-003)

摘　　要：目的了解吉林省2011年<5岁婴幼儿诺如病毒(Norovirus,NV)感染的流行病学特征及基因型。方法收集2011年长春市儿童医院<5岁住院腹泻患儿的粪便标本375份,采用逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)检测粪便标本中的NV。结果冬春季为NV感染发病高峰期;<2岁病例数占94.1%,临床症状以腹泻、呕吐和发热为主。375份粪便标本中,阳性检出率为13.6%,PCR检测全部为基因Ⅱ组(GenogroupⅡ,GⅡ)。选取8份阳性标本进行核苷酸序列分析,结果显示:NV GⅡ-4型5株,NV GⅡ-3型2株,NV GⅡ-13型1株。结论NV是引起<5岁婴幼儿病毒性腹泻的重要病原之一,住院病例中<2岁婴幼儿居多,GⅡ-4型略占优势。Objective To describe the epidemiological characteristics and genotype of norovirus （NV） infection in infants less than 5 years old in Jilin province. Methods 375 fecal specimens of diarrhea patients in infants were collected in Changcbun Children＇ s Hospital. We detected NV by reverse transcription-polymeraes chain reactions（RT-PCR）. Results For NV cases, there was a peak of occurrence during the cold season, children under 2 years accounted for 94.1% of all cases. And main clinical symptoms were diarrhea,vomiting and fever. 53.6% of 357 specimens were positive for NV ribonucleic acid. All of them belonged to genogroup II （GII） by RT-PCR. The sequence analysis showed that 5 strans belonged to genotype GII-4, 2 strans belonged to genotype GII -3 and 1 strans belonged to genotype GII-13. Conclusion NV is the one of the frequency of causing viral diarrhea in infants under 5 years in Jilin province. NV infection is clinically common in infants under 2 years old in hospital. Genotype GII -4 was the predominant genotype.

关 键 词：〈5岁婴幼儿 诺如病毒 基因型 流行病学特征 

分 类 号：R373.11[医药卫生—病原生物学] R183.3[医药卫生—基础医学]