酸碱胁迫对人参不定根中皂苷积累的影响及皂苷的组织化学定位研究  

Effects of acid and alkali stress on ginsenoside content and histochemical localization of ginsenoside in adventitious root of Panax ginseng

作　　者：李铁军[1] 廉美兰[1] 邵春绘[1] 于丹[1] 朴炫春[1] 

机构地区：[1]延边大学长白山生物资源与功能分子教育部重点实验室,吉林延吉133002

出　　处：《中国中药杂志》2013年第24期4277-4280,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81160497)

摘　　要：为了研究酸碱胁迫对人参不定根中皂苷含量的影响,不定根在反应器中培养30 d后进行酸碱处理的结果,酸碱处理可提高不定根中皂苷含量。用酸和碱处理后皂苷最高含量出现的时期不同,酸处理后的第5天人参皂苷质量分数达最高(14.0 mg·g-1DW),而碱处理在第7天出现皂苷质量分数的最高值(14.1 mg·g-1DW)。对不定根中皂苷进行组织化学定位的研究表明,不定根组织中皂苷的颜色呈现出浅红到深红的颜色变化,皂苷主要积累于中柱细胞。To investigate the effect of acid and alkali stress on ginsenoside content of Panax ginseng, adventitious roots culture in bioreactors were incubated for 30 d and pH value was adjusted. Ginsenoside content increased by reducing or raising the pH in culture medium, the muxium ginsenoside content was determined on the 5^th days after acid treatment and on the 7^th days after alkali treatment. The result of histochemical localization of ginsenoside revealed that the red color from light to dark were found in the adventitious root tissue, and ginsenoside mainly located in the pericycle cells where appeared the dark red color.

关 键 词：人参不定根 酸碱胁迫 解剖结构 组织化学定位 

分 类 号：S567.51[农业科学—中草药栽培]