靶向亲和-液质联用技术对人参茎叶总皂苷中α-葡萄糖苷酶抑制剂的快速筛选  

Screening and Structures Characterization of α-Glucosidase Inhibitors from Total Saponins of Ginseng Stems and Leaves by Ultrafiltration LC-MS^n

作　　者：何忠梅[1] 王晓慧[1] 李国峰[2] 孙佳明[3] 杨鹤[1] 郜玉钢[1] 张连学[1] 

机构地区：[1]吉林农业大学人参工程技术创新中心,长春130118 [2]长春中医药大学第一附属医院,长春130117 [3]长春中医药大学中医药与生物工程研究开发中心,长春130117

出　　处：《分析化学》2013年第11期1694-1698,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.31070316);国家科技支撑计划项目(No.2011BAI03B010602);吉林省科技条件与平台建设计划(No.20112101);国家科技重大专项子课题(No.2012ZX09304006)资助

摘　　要：建立了用于快速筛选人参茎叶总皂苷中α-葡萄糖苷酶抑制剂的靶向亲和-液相色谱-质谱联用技术(Target molecule affinity-LC-ESI-MSn)。从人参茎叶总皂苷中筛选并鉴定出了人参皂苷Rb3等12种具有潜在α-葡萄糖苷酶抑制活性的化合物,同时对其中5种化合物(包括人参皂苷F2,Rf,Rg3,Rd和Rb3)进行了活性验证,发现人参皂苷Rb3活性最强,对α-葡萄糖苷酶活性抑制率为43.16%,抑制作用接近总皂苷。本方法直接从复杂的中药提取物中筛选出具有抑制α-葡萄糖苷酶的活性成分,简单、快速、准确,适用于实现复杂体系中α-葡萄糖苷酶抑制剂的高通量筛选。An target molecule affinity-LC-ESI-MSn method was established to rapid screening α-glucosidase inhibitors from total saponins of ginseng stems and leaves. As a result, the inhibitory effects from twelve compounds including ginsenoside Rb3 presented α-glucosidase were observed and identified, meanwhile, five of them including ginsenoside F2, Rf, Rg3 , Rd and Rb3were done for activity validation. Rb3 showed the strongest activity for α-glucosidase, inhibition rate is 43.16%, close to total saponins. The method is simple, rapid, accurate and suitable for directly screened active ingredients which can inhibit α-glycosidase from complex extract of traditional Chinese medicines.

关 键 词：靶向亲和-液质联用技术 人参茎叶总皂苷 Α-葡萄糖苷酶抑制剂 

分 类 号：R284.1[医药卫生—中药学]