基于卵磷脂膜中铁卟啉直接电化学的过氧化氢传感器  

Hydrogen Peroxide Biosensor Based on Direct Electrochemistry of Hemin in Egg-Phosphatidylcholine Films

作　　者：马生华[1] 王雪靖[1] 韩晓军[1,2] 

机构地区：[1]哈尔滨工业大学化工学院,哈尔滨150001 [2]哈尔滨工业大学城市水资源与水环境国家重点实验室,哈尔滨150001

出　　处：《分析化学》2013年第11期1719-1723,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.21003032;21273059);电分析化学国家重点实验室开放基金(No.SKLEAC201313)资助

摘　　要：将蛋黄卵磷脂（egg-PC）和铁卟啉混合,浇筑到氧化铟锡玻璃（ITO）表面,实现了铁卟啉的直接电化学.结果表明,循环伏安图上出现了铁卟啉的一对峰形良好、对称的氧化还原峰.通过计算可以得到电活性铁卟啉的表面覆盖度为Γ=3.25×10-12 mol/cm2.随着磷酸盐缓冲溶液pH值从4.0升高到9.0,铁卟啉的式电位E°’线性负移,斜率为-43.7 mV/pH.综合考虑,在pH 4.0溶液中对H202检测,线性检测范围为10～ 200 μmol/L,检出限为5μmol/L.此修饰电极对H2 02具有良好的电催化活性,基于此研制出无媒介体的H2O2传感器.The mixture of egg-phosphatidylcholine （PC） vesicles and hemin was casted onto indium-tin oxide glass （ITO） electrode. The hemin showed a direct electrochemical behavior on the modified electrode. A well- defined pair of redox peaks was obtained from the cyclic vohammograms at the mixture of hemin and egg-PC modified ITO electrode. The average value of the surface coverage of electroactive hemin, F, was equal to 3.25×10-12 mol/cm2. The formal potential （E0） of hemin in the lipid film was linearly varied with the pH of phosphate buffer solution in the range from 4.0 to 9.0 with a slope of 43.7 mV/pH. The pH value of buffer solution for measuring hydrogen peroxide was decided to be 4. O. The linear range of our biosensor was from 10 μmol/L to 200 μmol/L with a detection limit of 5μmoL/L. The modified electrode exhibited a good catalytic activity towards hydrogen peroxide. Therefore a third generation biosensor was developed.

关 键 词：铁卟啉 蛋黄卵磷脂 传感器 过氧化氢 

分 类 号：O657.1[理学—分析化学]