高效液相色谱-串联质谱同时测定毛竹笋中4种内源性植物激素  

Determination of Four Endogenous Phytohormones in Bamboo Shoots by Liquid Chromatography-Tandem Mass Spectrometry

作　　者：钟冬莲[1] 丁明[1] 汤富彬[1] 莫润宏[1] 滕莹[1] 

机构地区：[1]中国林科院亚热带林业研究所,国家林业局经济林产品质量检验检测中心(杭州),富阳311400

出　　处：《分析化学》2013年第11期1739-1743,共5页Chinese Journal of Analytical Chemistry

摘　　要：建立了同时检测毛竹笋中赤霉素、脱落酸、吲哚-3-乙酸和吲哚-3-丁酸4种内源性植物激素的高效液相色谱-串联质谱（HPLC-MS/MS）方法.竹笋样品经甲醇-甲酸溶液（99∶1,V/V）提取后,C18固相萃取柱净化,采用反相C18色谱柱分离.以超纯水和甲醇溶液作为流动相进行梯度洗脱,采用多反应监测离子模式（MRM）进行定性分析,基质标准曲线外标法进行定量分析,线性范围在2～200μg/L之间.在5,50和200 μg/kg添加水平下,4种内源植物激素的回收率为75.2％～105.0％;相对标准偏差为0.8％ ～11.4％.本方法的定量限为0.1～ 1.0 μg/kg.A method was developed for the determination of four endogenous phytohormones, gibberellins, 3-indolebutytic acid, abscisic acid and indole-3-acetic acid in bamboo shoots by liquid chromatography-tandem mass spectrometry （LC-MS/MS）. The four analytes were extracted from bamboo shoots by methanol-formic acid （99： 1, V/V）, and purified by solid extraction using ClS sorbent, and then analyzed by liquid chromatography-tandem mass spectrometry （LC-MS/MS）. The four analytes were then separated on a reversed phase column with mobile phase containing water and methanol in a gradient elution mode. Qualitative analysis was performed using multiple-reaction monitoring （MRM）. Matrix-matched calibrations were respectively used to quantify the analytes concentrations. The recoveries were in the range of 75.2%-105.0% , and the relative standard deviations （RSDs） were 0.8% -11.4% at three spiked levels of 5, 50 and 200 μg/kg for all analytes. The limits of quantification （LOQ） for all analytes were between 0.1 and 1.0 μg/kg.

关 键 词：液相色谱-串联质谱法 内源植物激素 毛竹笋 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]