HPLC测定马钱子及其提取物中马钱子碱和士的宁含量  

Determination of Brucine and Strychnine in Semen Strychni and Its Extractive by HPLC

作　　者：刁国兰 张贵华[2] 马云淑[3] 张文平[3] 黄金娥[3] 罗红梅[3] 

机构地区：[1]四川省医药保健品进出口公司,四川成都610016 [2]云南省中医医院药剂科,云南昆明650021 [3]云南中医学院药学院,云南昆明650500

出　　处：《中国中医药信息杂志》2013年第12期51-53,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(30960517)

摘　　要：目的采用反相高效液相色谱法建立同时测定马钱子药材及其总碱提取物中马钱子碱和士的宁含量的方法。方法采用Licrospherc18色谱柱（4．6mm×250mm，5μm），流动相为乙腈：0．01mol／mL庚烷磺酸钠与0．02mol／mL磷酸二氢钾等体积混合溶液（用10％磷酸调pH值为2．8）=27：73，检测波长260nm，柱温30℃，流速为1mL／min。结果马钱子碱在0．1—1．0μg、士的宁在0．12～1．2μg范围内线性关系良好（r=1．000），平均回收率分别为99．88％（RSD=1．06％）、100．060（RSD=0．78％）。结论本方法操作可靠、精确，可用于中药马钱子药材及提取物的含量测定。Objective To develop a RP-HPLC method for the determination of Brucine and Strychnine in Semen Strychni and its extractive of total alkaloids. Methods A chromatographic column of Licrospher C18 （4.6mm×250mm, 5 μm） was used with the mobile phase of acetonitrile ： 0.01 mol/L sodium heptane sulfonate and 0.02 mol/L potassium dihydrogen phosphate mixed with equal amount {adjusted pH to 2.8 with 10% phosphonic acid）=27 ; 73, detection wavelength at 260 nm, column temperature of 30 ℃ and flow rate of 1 mL/min. Results The calibration curves of Brucine and Strychnine were both in good linearity in the ranges of 0.1-1.0 μg and 0.12-1.2 μg （r=1.000） respectively. The average recovery rates of Brucine and Strychnine were 99.88% （RSD=1.06%） and 100.06% （RSD = 0.78%） respectively. Conclusion The method is realiable and accurate, which can be applied to determination of Brucine and Strychnine in Semen Strychi and its extractive.

关 键 词：马钱子 马钱子碱 士的宁 含量测定 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]