HPLC测定药用芦荟中芦荟苷和芦荟大黄素含量  

HPLC Determination of Aloin and Aloeemodin in Barbodos Aloe

作　　者：彭贵龙[1] 周光明[1] 秦红英[1] 

机构地区：[1]西南大学化学化工学院发光与实时分析教育部重点实验室,重庆400715

出　　处：《中国中医药信息杂志》2013年第12期59-61,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(21277110)

摘　　要：目的以离子液体1-丁基-3-甲基咪唑溴化盐（[BMIM]Br）为萃取剂超声辅助萃取，高效液相色谱法同时分离测定芦荟中的芦荟苷和芦荟大黄素。方法采用PhenomenexC18色谱柱（250mm×4．6mm，5μm）；流动相：甲醇-0．3％冰醋酸水溶液（65：35）；流速：0．80mL／min；紫外检测波长：360nm；柱温：35℃。结果芦荟苷和芦荟大黄素分别在0．000336-1．68lag（r=0．99996）、0．000608-3．04μg（r=0．99976）范围内线性关系良好，检出限分别为0．0506、0．262ng／mL，平均加样回收率分别为95．99％、95．80％。结论本法操作简单快速、定量准确、灵敏度高、成本低，且对环境友好，是检测及分离芦荟中蒽醌类化合物的有效方法。Objective To isolate and determine Aloin and Aloeemodin in Barbodos Aloe by ionic liquid-based ultrasonic-assisted extraction coupled with high performance liquid chromatography, with 1-butyl-3-methylimidazolium chloride （[BMIM]Br） solution as the extraction solvent. Methods The separation was performed on Phenomenex C18 column （250 mm×4.6 mm, 5 μm） with detection wavelength of 360 nm. The mobile phase was consisted of methanol-0.3% acetic acid solution （65 ： 35） with the flow rate of 0.80 mL/min, and the column temperature at 35 ℃. Results The calibration curves for Aloin and Aloeemodin were liner within 0.000 336-1.68μg （r=0.999 96） and 0.000 608-3.04 μg （r= 0.999 76）, respectively. The limit of detection （LOD） was 0.05 06 ng/mL and 0.262 ng/mL, respectively. The average recovery was 95.99% and 95.80%, respectively. Conclusion The method is simple, rapid, accurate, sensitive, low cost and environment-friendly, thus it provides an effective means for assaying anthraquinones in Barbodos Aloe.

关 键 词：离子液体 超声辅助 高效液相色谱法 芦荟 芦荟苷 芦荟大黄素 

分 类 号：R284.1[医药卫生—中药学]