桂花组织培养快繁体系的建立  

In Vitro Culture and Rapid Propagation of Osmanthus fragrans

作　　者：李林[1] 韩远记[1] 袁王俊[1] 董美芳[1] 尚富德[1] 

机构地区：[1]河南大学生命科学学院,河南开封475004

出　　处：《河南大学学报（自然科学版）》2013年第6期667-671,共5页Journal of Henan University:Natural Science

基　　金：国家自然科学基金(31270738);河南省杰出青年基金(094100510018)联合资助

摘　　要：以潢川金桂的嫩枝茎尖为外植体,进行离体培养与快速繁殖研究.结果显示:桂花嫩枝茎尖的最佳消毒处理为0.1%升汞处理2.5min;最适初代培养基为B5+2.5mg/L 6-BA+0.05mg/L NAA+3%蔗糖;最适继代培养基为B5+2.0mg/L 6-BA+0.05mg/L NAA+3%蔗糖;最适生根条件为1/2MS+2.0mg/L NAA+全黑暗处理;采用营养土为移栽基质.经过实验,发现桂花从一个茎尖到成苗移栽只需4个月左右的时间,30d时增殖系数达到2.08,移栽成活率高达82%.To study the tissue culture of Osmanthus fragrans in various periods of optimal conditions, and provide technical support for large-scale, industrialized production of Osmanthus fragrans, using the shoot tip of ＇Huangchuan Jingui＇ as the explants, in vitro culture and high frequency propagation of sweet osmanthus were studied. The results showed shoot tips of sweet-scented osmanthus twigs disinfection processing 2.5 min, 0.1 HgCl2, the most suitable initial media were Bs ＋2.5 mg/L 6-BA＋0.05 mg/L NAA＋ 3% sucrose, the most suitable regeneration medium was Bs ＋ 2.0 mg/L 6-BA ＋ 0.05 mg/L NAA＋3% sucrose, the most conducive rooting medium was 1/2 MS＋2.0 mg/L NAA＋ full of darkness. Using nutritive soil as transplanting matrix, the authors found that it was about four months from a shoot tip to transplanting for ＇ Huangchuan Jingui＇, the multiplication coefficient reached 2.08 used 30 d, and the survival percentage of plantlets could reach 82%.

关 键 词：桂花 玉米素 离体培养 快速繁殖 

分 类 号：Q943.1[生物学—植物学]