珍禽褐马鸡线粒体DNA控制区结构和亲缘关系  

The structure of mitochondrial DNA control region and the phylogenetic relationship of brown-eared pheasant( Crossoptilon mantchuricum ).

作　　者：武玉珍[1] 冯睿芝[2] 张峰[3] 

机构地区：[1]晋中学院生物科学与技术学院,山西晋中030600 [2]复旦大学生物医学研究院,上海200032 [3]山西大学生命科学院,太原030006

出　　处：《生态学杂志》2013年第12期3243-3249,共7页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(31040018);科技部科技基础性工作专项(2011FY110300);山西省自然科学基金项目(2012011034-3);山西省回国留学人员科研项目(20100012);山西省教改项目(J2011088)资助

摘　　要：为了解褐马鸡种群的遗传多样性水平，确定种群之间和个体之间的亲缘关系，本文采用聚合酶链式反应和直接测序的方法，对野生和圈养两个种群20只褐马鸡线粒体DNA控制区全序列进行了测定。对比其他报道的雉类线粒体控制区结构，分析了褐马鸡线粒体DNA控制区结构，识别了其高变Ⅲ区、中间保守Ⅱ区和保守I区。使用邻接法和最大简约法建立的系统树显示：褐马鸡2个种群个体之间的遗传距离在0—0．009，平均值为0．002；2个种群间的基因流数值Wm=4．67。结果表明：褐马鸡线粒体DNA控制区结构与其他鸟类相似，2个种群之间以及种群内个体之间的遗传距离较小、亲缘关系较近。本研究可为合理制定褐马鸡的圈养及野外放归计划提供科学依据。Brown-eared pheasant （ Crossoptilon mantchuricum） is an endemic and first-class pro- tected bird in China, which only distributes separately in North China. In order to understand the genetic diversity of C. mantchuricum populations and the phylogenetic relationships between the C. mantchuricum populations and between the individuals, the polymerase chain reaction （PCR） and sequencing were adopted to determine the sequence of the mitochondrial DNA control region of 20 brown-eared pheasants from the Pangquangou Nature Reserve and Taiyuan Zoo in Shanxi Province of China, respectively. In comparing with the known structure of mitochondrial DNA control region of the other pheasants, the structure of the mitochondrial DNA control region of brown-eared pheasant was analyzed, and the hyper-variable region III, medium conservative re- gion II, and conservative region I were identified. The phylogenetic trees were constructed with neighbor-joining （NJ） method and maximum-parsimony （MP） method. The genetic distances of the individuals between the two populations were from 0 to 0. 009, with a mean value of 0.002. The genetic flow between the two populations （Nm） was 4.67. Overall, the structure of the mito- chondrial DNA control region of the brown-eared pheasant was similar to that of the other pheasa- nts. The genetic distances between the two populations and between the individuals were smaller, and the genetic relationships were closer. This study could provide scientific evidence for the rea- sonable management of captive brown-eared pheasant and for the eventual reintroduction of the captive brown-eared pheasant to the wild.

关 键 词：褐马鸡 线粒体DNA 结构 亲缘关系 

分 类 号：Q953[生物学—动物学]