N-组氨酸壳聚糖多孔支架对牛血清白蛋白的吸附性能  

Adsorption Behavior of Bovine Serum Albumin by Histidine Grafted Chitosan Porous Scaffolds

作　　者：陈彰旭[1,2] 辛梅华[1] 李明春[1] 朱志梅[2] 陈晓东[1] 

机构地区：[1]华侨大学材料利学与工程学院,福建厦门361021 [2]莆田学院环境与生物工程学院,福建莆田351100

出　　处：《高分子材料科学与工程》2013年第12期107-111,共5页Polymer Materials Science & Engineering

基　　金：国家自然基金青年基金资助项目(21103095);福建省自然科学基金资助项目(2011J01312,2012J01396);福建省高校服务海西建设重点项目(2009HX11)

摘　　要：通过改变组氨酸/壳聚糖的配比及壳聚糖的相对分子质量合成了6种N-组氨酸壳聚糖(NHCS)多孔支架,考察其在模拟体液中吸附牛血清白蛋白(BSA)的能力,用紫外-可见分光光度法测定NHCS不同温度的吸附等温线和不同起始浓度下的吸附动力学曲线等。结果表明,在37.0℃、BSA的初始浓度为2.5 mg/mL时,壳聚糖的相对分子质量为50×103、组氨酸/壳聚糖的摩尔比为3∶1制得的50kD-NHCS-3多孔支架吸附BSA效果最好,吸附容量(Qe)为820.90 mg/g;吸附遵循Langmuir吸附等温式和准一级动力学方程,且是一个自发、熵增、吸热的物理吸附过程。50kD-NHCS-3多孔支架可以重复使用,有望用于BSA或其他蛋白的分离纯化。Six type of histidine grafted chitosan （NHCS） porous scaffolds were synthesized by changing the mole ratio of histidine/chitosan and the molecular weight of the chitosan. Adsorption behavior of bovine serum albumin （BSA） by NHCS in simulated body fluid （SBF） was investigated. Ultraviolet-visible spectrophotometer was used to determine the adsorptive equilibrium curve at different initial concentrations and the adsorptive dynamic curve at different temperatures. The results indicate the adsorption capacity of 50kD-NHCS-3 （S4, Mw（CS） is 50 103, n （histidine）/n （chitosan） is 3：1） is the best of all studied NHCS porous scaffolds, the maximum adsorption capacity is 820.90 mg/g at 37.0℃, while the initial concentration of BSA solution is 2.5 mg/mL, The adsorption kinetics is in good agreement with pseudo first-order kinetic model, whereas, the adsorption isotherms could be well described by Langmuir equation. The adsorption of BSA is a spontaneous, entropy increasing, endothermic and physical process. 50kD-NHCS-3 is a good reusable matrix for adsorption and separation of BSA.

关 键 词：N-组氨酸壳聚糖 多孔支架 牛血清白蛋白 吸附 

分 类 号：O636.1[理学—高分子化学]