宫内膜细胞p16基因表达检测筛查子宫内膜癌的研究  

The study on expression of p16 gene in endometrial cell for screening of endometrial carcinoma

作　　者：曹晓智[1] 王小捷[1] 李凤山[2] 

机构地区：[1]皖南医学院第二附属医院病理科,安徽芜湖241000 [2]南京医科大学附属南京市妇幼保健院病理科,江苏南京210004

出　　处：《中国计划生育和妇产科》2013年第6期47-49,共3页Chinese Journal of Family Planning & Gynecotokology

摘　　要：目的研究良性病变子宫内膜细胞与子宫内膜癌细胞中p16基因的表达,探讨p16基因表达检测筛查子宫内膜癌的可行性及临床应用价值。方法使用子宫内膜细胞采集器采集子宫内膜细胞,采用实时荧光定量逆转录聚合酶链反应(Real-time Quantitative Reverse Transcription Polymerase Chain Reaction,RT-PCR)检测51例子宫内膜细胞标本中的p16基因mRNA,其中良性病变子宫内膜细胞16例,子宫内膜癌细胞35例,比较两者间基因表达的差异;统计p16基因表达检测筛查子宫内膜癌的敏感性和特异性。结果 16例良性病变子宫内膜细胞标本p16基因mRNA水平显著高于35例子宫内膜癌细胞标本(P=0.027),宫腔细胞p16基因表达检测诊断子宫内膜癌的敏感性为73.7%,特异性为78.9%。结论宫内膜细胞p16基因表达检测适用于门诊及大规模子宫内膜癌的筛查,尤其适用于症状人群及高危人群的常规筛查。Objective To investigate the expression of p16 gene between cells of endometrial benign lesion and endometrial carcinoma,to discuss the feasibility and clinical value of p16 gene expression in endometrial cell as a screening method for the diagnosis of endometrial carcinoma. Methods Endometrial cells were obtained from 51 patients, including 16 cases of endometrial benign lesion and 35 cases of endometrial carcinoma. Real -time Quantitative Reverse Transcription Polymerase Chain Reaction （QRT- PCR ） were performed to examine the expression of p16 gene between two groups. Calculate the sensitivity and specificity of p16 gene expression for screening of endometrial carcinoma. Results p16 gene mRNA was significantly higher in benign endometrial ceils than which in endometrial carcinoma （ P = 0. 027 ）. The examination of p16 gene expression in endometrial cells demonstrated a sensitivity of 73.7% and specificity of 78. 9% for screening endometrial carcinoma. Conclusion The examination of p16 gene expression in endometrial cells is applicable for outpatient and screening endometrial carcinoma in large - scale, especially suitable for screening endometrial carcinoma in symptoms crowd and high - risk population.

关 键 词：子宫内膜癌 筛查 P16基因 实时荧光定量逆转录聚合酶链反应 

分 类 号：R714.2[医药卫生—妇产科学]