植物激素对黄瓜愈伤组织形成与芽分化的影响  

Effects of Phytohormones on Callus Formation and Buds Differentiation of Cucumber

作　　者：王萍[1] 沈晓婷 王罡[3] 季静[3] 

机构地区：[1]淮海工学院海洋学院,江苏连云港222005 [2]古蔺县职业高级中学校,四川古蔺646500 [3]天津大学环境科学与工程学院,天津300072

出　　处：《北方园艺》2013年第24期84-87,共4页Northern Horticulture

基　　金：国家自然科学基金资助项目(31271419);中央财政支持地方高校发展专项资金资助项目(CXTD07)

摘　　要：以黄瓜“津研四号”子叶节为外植体，采用L9（3^4）正交实验设计，在MS基本培养基中添加6-BA、IAA、ABA、KT4种植物激素，研究不同激素组合对黄瓜子叶节组织培养芽分化与伸长的影响。结果表明：在MS培养基中添加4种激素中的任何3种均能形成愈伤组织，添加1～2mg／L6-BA＋0．5mg／LABA＋4mg／LKT（或0．050mg／LIAA）有利于芽分化，添加2mg／L6-BA＋0．025mg／LIAA＋4mg／LKT可促进芽的伸长；在培养2周时6-BA、IAA、ABA对再生频率、芽数、芽长的作用值相似，均大于KT的作用；在培养6周时对再生频率、芽数、芽长影响较大的激素是6-BA和ABA；ABA可促进黄瓜芽分化，但在培养6周时抑制芽的伸长。Using cotyledonary nodes of ＇ Jinyan 4 ＇ as explants, the orthogonal experiment design Ls （34 ） was applied to investigate the effects of phytohormones combination on differentiation and elongation of buds with MS basic medium plus 4 phytohormones （6-BA,IAA,ABA,KT） in cucumber. The results showed that MS medium plus any three o{ four phytohormones could form callus,MS medium plus 1-2 mg/L 6-BA,0. 5 mg/L ABA and 4 mg/L KT （or 0. 050 mg/L IAA） could facilitate the differentiation of adventitious buds obviously in cucumber, whereas, MS medium plus 2 mg/L 6-BA,0. 025 mg/L IAA and 4 mg/L KT was beneficial for the elongation of buds. The effects of 6-BA,IAA,ABA on regeneration rates, number of buds and length of buds were similar and more evident than those of KT when the explants were cultured for 2 weeks. Among these 4 phytohormones, 6-BA and ABA were demonstrated to be more effective on regeneration rates,number of buds and length of buds relatively when the explants were cultured for 6 weeks. Although ABA could promote of the differentiation of adventitious buds in cucumber,it could suppress the elongation of buds when the explants were cultured for 6 weeks.

关 键 词：黄瓜 植物激素 子叶节 组织培养 

分 类 号：S642.2[农业科学—蔬菜学]