不同激素对青花菜下胚轴不定芽分化的影响  

Influence of Different Hormones on Broccoli Hypocotyl Adventitious Bud Differentiation

作　　者：于娅[1] 于亚萍[1] 段威[1] 

机构地区：[1]武汉生物工程学院生物工程系,湖北武汉430415

出　　处：《北方园艺》2013年第24期96-99,共4页Northern Horticulture

基　　金：湖北省教育厅科研资助项目(B20104602)

摘　　要：以"碧绿2号"青花菜为试材,以下胚轴为外植体,研究了不同浓度的TDZ、6-BA、ZT对青花菜下胚轴外植体不定芽分化及下胚轴上部、中部、下部不同部位对不定芽分化的影响。结果表明:青花菜下胚轴不定芽分化最佳培养基直观组合为MS+0.2mg/L NAA+0.5mg/L ZT+0.2mg/L TDZ+1.0mg/L 6-BA+3%蔗糖+0.8%琼脂;下胚轴的上部不定芽分化率最高,达90%。With broccoli variety ＇ Bilv No. 2 ＇ as material, taking the hypocotyl as explants, the influence of TDZ, 6-BA, ZT and different concentrations of plant growth regulators on its adventitious buds of hypocotyls,and the effects of different explants of upper middle and lower parts on adventitious buds differences were studied. The results showed that the best medium was MS＋0. 2 mg/L NAA＋0. 5 mg/L ZT＋0. 2 mg/L TDZ＋I mg/L 6-BA＋3% sucrose＋0. 8% agar;upper parts of hypocotyls had highest rate,it was 90%.

关 键 词：青花菜 下胚轴 不定芽 分化 

分 类 号：S635.3[农业科学—蔬菜学]