苦瓜MAP30基因的密码子优化及在毕赤酵母中的表达  

Codon Optimization and Expression of MAP30 in Pichia pastoris Expression System

作　　者：王芳[1] 王丽媛[1] 乔禹[1] 韩旭[1] 丁国华[1] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院,黑龙江省高等院校植物学重点实验室,黑龙江哈尔滨150025

出　　处：《北方园艺》2013年第24期112-115,共4页Northern Horticulture

基　　金：黑龙江省教育厅科研资助项目(11551142);黑龙江省高校科技创新团队研究计划资助项目;哈尔滨师范大学科技创新团队研究计划资助项目(KJTD-2011-2)

摘　　要：以苦瓜为试材,采用SDS-PAGE和Western-blot的方法,研究经密码子优化后的苦瓜MAP30(Momordica anti-HIV protein of 30kDa)基因在新型毕赤酵母中的表达。结果表明:与未优化基因相比,优化基因在新型毕赤酵母中的表达量有所提高。pGAPHα与甲醇诱导型启动子(pPIC9)相比提高了安全性,适合应用到医药领域。Taking Momordica charantia as experimental charantia in Pichia pastoris expression was studied by expression quantity of the optimized MAP30 gene was system, pGAPHa vector composed of GAP promotor was material, the codon optimized MAP30 gene of Momordica SDS-PAGE and Western-blot. The results showed that the increased slightly in the neotype Pichia pastoris expression safer than pPIC9 composed of AOX promotor. It is supposed that the new way should be used in medical field.

关 键 词：苦瓜 M-AP30 毕赤酵母pGAPHα 优化基因 

分 类 号：S642.5[农业科学—蔬菜学]