纳米铂与牛血清白蛋白相互作用的光谱研究  

Investigation of Interaction between Platinum Nanoparticles and Bovine Serum Albumin by Spectroscopy

作　　者：辛建伟[1] 马红燕[1] 高春燕[1] 吴坚[1] 

机构地区：[1]延安大学化学与化工学院分析化学研究所,陕西延安716000

出　　处：《分析科学学报》2013年第6期835-838,共4页Journal of Analytical Science

基　　金：陕西省教育厅科研基金(No.12JK0616);延安大学科研基金(No.YDZ2012-10);国家级大学生创新创业训练计划(No.21103146)

摘　　要：应用荧光光谱法和紫外-可见吸收光谱法研究了纳米铂(PtNP)与牛血清白蛋白(BSA)之间的相互作用。研究发现,PtNP与BSA猝灭机制为静态猝灭。求得了不同温度下PtNP与BSA之间相互作用的结合常数K≈104,结合位点数n≈1,根据热力学参数值确定了它们之间的作用力为疏水作用力。同时应用同步荧光光谱考察了PtNP对BSA构象的影响。The interaction between platinum manoparticles（PtNP） and bovine serum albumin（BSA） was studied by fluorescence spectroscopy and UV-Vis absorption spectroscopy. The results showed that PtNP caused the fluorescence quenching of BSA through a static quenching procedure. The binding constant（K~ 104） and binding site （n=1） between PtNP and BSA were obtained. According to the values of thermodynamic functions, the type of force between PtNP and BSA was hydrophobic interaction. At the same time,the effect of PtNP on the conformation of BSA was investigated by using synchronous fluorescence spectroscopy.

关 键 词：纳米铂 牛血清白蛋白 荧光光谱 紫外-可见吸收光谱 

分 类 号：O657.39[理学—分析化学]