藏红花花瓣体外抗氧化活性部位的筛选与研究  

Bioactivity-guided Fractionation of Petals of Crocus Sativusand Their Antioxidant Activity Investigation

作　　者：黄一泓[1] 刘雅莉[1] 武文斌[1] 张雯[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《天然产物研究与开发》2013年第11期1489-1493,1567,共6页Natural Product Research and Development

基　　金：国家自然科学基金项目(61172149)

摘　　要：采用有机溶剂萃取法分离藏红花花瓣乙醇提取物,分别得到石油醚相、氯仿相、乙酸乙酯相、正丁醇相和水相5个不同极性部位,以总抗氧化能力(T-AOC)及对羟自由基(·OH)、超氧阴离子自由基(O-·2)和2,2-二(4-叔辛基苯基)-1-苦肼基自由基(DPPH·)的清除能力为评价指标初步筛选其活性部位,并对活性最强部位进行了脂质抗氧化研究。结果显示,藏红花花瓣提取物不同极性部位均能明显清除上述自由基,其中乙酸乙酯相的清除能力最强。在进一步的脂质抗氧化研究中,10 mg/mL乙酸乙酯相对红细胞自氧化及H2O2诱导的过氧化溶血的抑制率分别达到40.36%和68.82%;对小鼠肝脏自氧化及H2O2诱导的过氧化所产生的MDA的抑制率分别为39.81%和47.53%。实验结果表明:藏红花花瓣不同极性部位提取物均有不同程度的抗氧化活性,其中以乙酸乙酯相的抗氧化能力最强。In this study ,various fractions isolated from the petals of Crocus sativus were assessed for their antioxidant activities. Five extracts were obtained by using different polarity solvents ,including petroleum ether, chloroform, ethyl ace- tate and n-butanol. The antioxidant activities of the five fractions were evaluated by the scavenging power of three free radicals, namely DPPH · , ·OH, O2· and T-AOC. The results indicated that the five fractions had strong scavenging capacity on DPPH · ,· OH and O2· , especially the ethyl acetate fraction （EA）. Hence, effect of EA on anti-lipid peroxidation was investigated. In addition, the protective effects on hemolysis of erythrocytes and lipid peroxidation of mice homogenate were measured by in vitro system. The inhibition of EA on hemolysis and lipid peroxidation were 40.36% and 39.81% ;the inhibition of EA on hemolysis and lipid perexidation induced by H202 were 68.82% and 47.53% when EA concentration was 10 mg/mL. According to data obtained from these antioxidant tests ,the ethyl acetate fraction demonstrated the strongest antioxidant capacity.

关 键 词：藏红花花瓣 自由基 乙酸乙酯提取物 脂质过氧化 

分 类 号：Q946.889[生物学—植物学]