大孔树脂吸附分离乌药叶总黄酮的研究  

Study on Adsorption and Separation of Total Flavonoids from Lindera aggregata Leaves by Macroporous Resin

作　　者：许海丹[1] 朱威[2] 赵波[1] 顾霞敏[1] 

机构地区：[1]台州学院医药化工学院,台州318000 [2]浙江大学工业技术研究院,杭州310018

出　　处：《天然产物研究与开发》2013年第11期1545-1549,1571,共6页Natural Product Research and Development

基　　金：浙江省分析测试科技计划项目(2008F70008)

摘　　要：通过比较8种大孔吸附树脂对乌药叶总黄酮的吸附分离性能,筛选出适合分离乌药叶总黄酮的树脂,并对其动态吸附特性进行研究。结果表明D101树脂对乌药叶黄酮有良好的吸附和分离性能,吸附量大,解吸率高,适合乌药叶黄酮的分离富集。该树脂分离乌药叶黄酮的工艺参数为:以2 BV/h流速上样,解吸前先用3BV水除杂,再用3BV、70%乙醇以2 BV/h的流速洗脱,解吸效果较好。经D101树脂吸附富集后,样品总黄酮含量达到了47.48%,是粗提物(11.05%)的4.3倍。D101大孔吸附树脂适合用于乌药叶黄酮的吸附分离,且其吸附平衡数据符合Langmuir和Freundlich等温吸附模型。In this study,eight types of macroporous resins were selected for adsorption and separation of total flavonoids from Lindera aggregata leaves. The results showed that D101 resin was an excellent adsorbent with higher adsorption and desorption capacity. The purification conditions of the investigated macroporous resin were also studied and optimized u- sing the static-dynamic adsorption and desorption characteristics. The results showed that the optimal adsorption condi- tions of D101 resin were as follows：2 BV/h as sampling flow rate,removing impurities by 3 BV water and eluting the to- tal flavonoids with 3 BV of 70% ethanol-water at flow rate of 2 BV/h. The content of total flavonoids of the product after the adsorption concentrating on D101 resin achieved 47.48% ,which was 4.3 times of crude extract （ 11.05% ）. Macro- porous resin D101 was suitable to separate and purify the total flavonoids from Lindera aggregata leaves. The adsorption behavior of D101 was proved to consistent with the Langmuir and Freundlich isotherms.

关 键 词：乌药叶 总黄酮 大孔树脂 吸附 分离 

分 类 号：R284.2[医药卫生—中药学]