偶氮胂Ⅲ-La(Ⅲ)光谱探针褪色光度法测定蒙药有效成分荜茇宁  

Fading spectrophotometric method determination of Piperlonguminine in Piperlongum L.by ArsenazoⅢ-La(Ⅲ) as spectral probe

作　　者：苗澍[1] 敖登高娃[1] 领小[1] 赵文岩[1] 

机构地区：[1]内蒙古大学化学化工学院,内蒙古呼和浩特010021

出　　处：《化学研究与应用》2013年第12期1693-1696,共4页Chemical Research and Application

基　　金：国家自然科学基金项目(81060366)资助;内蒙古自治区教育厅重点项目(NJZZ11014)资助;内蒙古自治区研究生科研创新资助项目资助

摘　　要：建立了一种简便快速的褪色分光光度法测定蒙药荜茇有效成分荜茇宁的方法。在酸性Britton．Robinson缓冲溶液中偶氮胂Ⅲ-La（Ⅲ）络合物在651nm处有最大吸收，络合物组成为1：1；加入荜茇宁形成组成比为1：1：2络合物而使溶液褪色，表观摩尔吸光系数1．203×10^5L·mol^-1·cm^-1，体系吸光度的降低值与加入药物质量浓度在0．0273—0．5466μg·mL^-1内服从朗伯比尔定律，回归方程为：AA=0．9653p＋0．0232（ρ：μg·mL^-1），检出限为0．02206μg·mL^-1。初步探讨了反应机理，并利用本方法对生物样品中的荜茇宁进行加标回收，回收率为99．8％～100．4％，相对标准偏差（RSD）为0．2—0．6％。A convenient and delicacy fading spectrophotometric method for the determination of piporlonguminine which is one of the Piperlongum L. effective components was established in the present work. In acidity tris-HCl buffer medium, the complex made up of 1 ： 1 Arsenazo Ⅲ-La（Ⅲ） has maximum absorption at 651 rim. Piperlonguminine could react with the complex that caused the fading of the Arsenazo Ⅲ and La（Ⅲ） solution. The composition ratio of Arsenazo Ⅲ-La（Ⅲ） and piperlonguminine was 1：1：2, and the apparent molar absorptivity was 1. 203×10^5 L·mol^-1·cm^-1. Beer＇s law was obeyed in the range of 0. 0273 - 0. 5466μg·mL^-1 of pip- erlonguminine and the regression equation was AA = 0. 9653p＋0. 0232 （ρ： μg·mL^-1 ） with the detection limit of 0. 02206 μg·mL^-1. Then the mechanism of reaction was discussed preliminarily. The proposed Fading spectrophotometric method was applied to the biological and urine samples spiked with standard piperlonguminine solution. The recovery of the samples was within the range of 99. 8% - 100. 4% and relative standard deviation（RSD） was 0. 2 - 0. 6%.

关 键 词：偶氮胂Ⅲ La(Ⅲ) 荜茇宁 褪色分光光度法 

分 类 号：O657.3[理学—分析化学]