乙烯利对小鼠脾脏和胸腺细胞周期及淋巴细胞亚群的影响  

Effects of ethephon on cell cycle and lymphocyte subsets of spleen and thymus in mice

作　　者：杨治峰[1,2] 薄存香[1] 刘永霞[1] 谢琳[1] 戈扬[1] 张振玲[1] 

机构地区：[1]山东省医学科学院山东省职业卫生与职业病防治研究院,山东济南250062 [2]济南大学山东省医学科学院医学与生命科学学院

出　　处：《环境与健康杂志》2013年第11期963-966,共4页Journal of Environment and Health

基　　金：山东省自然科学基金(ZR2010CL003)

摘　　要：目的利用流式细胞仪研究乙烯利对小鼠胸腺和脾脏细胞周期及淋巴细胞亚群的影响。方法将24只健康成年SPF级雄性昆明小鼠随机分为4组,分别为阴性对照(0 mg/kg)组和低(1/32 LD50,85 mg/kg)、中(1/16 LD50,170 mg/kg)和高剂量(1/8 LD50,340 mg/kg)组,每组6只。灌胃染毒,染毒容量为20 ml/kg,每天1次,连续染毒14 d。于首次给药后第28天,采用流式细胞术对小鼠脾细胞和胸腺细胞进行细胞周期检测,并观察对脾脏淋巴细胞CD3+、CD4+、CD8+及胸腺淋巴细胞CD4+、CD8+比例的影响。结果随着乙烯利染毒剂量的增加,小鼠脾细胞和胸腺细胞的细胞增殖受到了抑制。与阴性对照组比较,高剂量组小鼠脾脏淋巴细胞CD3+比例明显升高(P<0.05);各剂量组小鼠胸腺淋巴细胞CD8+比例明显升高(P<0.05),CD4+/CD8+比值下降(P<0.05);中、高剂量组小鼠胸腺淋巴细胞CD4+比例升高(P<0.05)。结论乙烯利对小鼠脾脏和胸腺淋巴细胞亚型比例有一定的影响,并通过对小鼠脾细胞和胸腺细胞的细胞周期阻滞作用,抑制了小鼠的免疫功能。Objective To study the effects of ethephon on cell cycle and lymphocyte subsets of thymus and spleen in mice by flow cytometry （FCM）. Methods Twenty-four SPF health adult Kunming male mice were randomly divided into four groups, control （0 mg/kg body weight）,low （85 mg/kg body weight） , moderate （170 mg/kg body weight） and high （340 mg/kg body weight） dose groups. The mice were treated with ethephon by gavage （20 ml/kg body weight）,once a day for 14 consecutive days. The thymus and spleen were collected at the 28th day after the first gavage. FCM was used to study the cell cycle progression of thymus and spleen cells,and to observe the percentage of CD3 ＋, CD4 ＋,CD8＋ lymphocytes of spleen and the percentage of CD4＋, CD8＋ lymphocytes of thymus. Results The cell cycle progression of immunocyte cells of mice spleen and thymus were blocked as the ethephon exposure dose increased. Compared with the control group,the number of CD3＋ T cell of the mice spleen of high dose groups increased （P〈0.05）, the number of CD8＋ T cell of the mice thymus increased （P〈0.05）,the number of CD4＋/CD8＋ ratio of the mice thymus decreased （P〈0.05）, the number of CD4＋ T cell of the mice thymus of moderate and high dose groups increased （P〈0.05）. Conclusion Ethephon may affect the immunological cell percentage of thymus and spleen in mice, and may block the process of immunocyte cell cycle.

关 键 词：乙烯利 胸腺 脾脏 淋巴细胞亚群 细胞周期 

分 类 号：R994.6[医药卫生—毒理学]