枸杞多糖对大鼠海绵体神经钳夹损伤的修复作用  

Neuroprotective effect of lycium barbarum polysaccharides after cavernous nerve crush injury in a rat model

作　　者：刘波[1,2] 丁协刚[1] 赵颀涵[3] 罗琼[3] 李世文[1] 王行环[1] 

机构地区：[1]武汉大学中南医院泌尿外科,430071 [2]湖北医药学院附属太和医院泌尿外科 [3]武汉大学公共卫生学院营养学系

出　　处：《中华实验外科杂志》2013年第12期2560-2562,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（303163398）;武汉大学自主科研项目（303275875）

摘　　要：目的 探讨使用枸杞多糖（LBP）修复钳夹损伤的海绵体神经（CN）,恢复自主勃起功能的可行性.方法 21只雄性SD大鼠随机分成3组,即假手术组、LBP组、损伤对照组,于术后3个月电刺激CN测定海绵体内压（ICP）及平均动脉压（MAP）,取CN进行甲苯胺蓝染色检测有髓轴突数目,取阴茎组织进行烟酰胺腺嘌呤二核苷酸磷酸（NADPH）染色检测一氧化氮合酶（NOS）阳性神经纤维数目.结果 LBP组ICP/MAP比值（0.44 ±0.05）明显高于损伤对照组（0.26 ±0.03,P＜0.05）,但低于假手术组（0.68 ±0.08,P＜0.05）.LBP组CN有髓轴突数目（106.8 ±17.9）,与假手术组（180.9 ±15.3）和损伤对照组（64.9±l3.9）比较差异有统计学意义（P＜0.05）.阴茎背神经NOS阳性神经纤维数目（164.2±12.2）,与假手术组（263.3 ±15.0）和损伤对照组（85.6±14.7）比较差异有统计学意义（P＜0.05）.结论 LBP可促进CN钳夹损伤后的神经再生及勃起功能的恢复.Objective To investigate the feasibility of erectile function recovery by repairing crushed cavernous nerves （CN） with lyceum barbarum polysaccharides （LBP）.Methods Twenty-one male Sprague-Dawley rats were randomly divided into 3 groups：sham group,LBP group,and control group.Erectile function was assessed by cavernous nerve electrostimulation at 3rd month after operation and nerve regeneration was assessed by toluidine blue staining of CN and nicotinamide adenine dinucleotide phosphate （NADPH）-diaphorase staining of penile tissue.Results The peak ICP/MAP ratio in LBP group （0.44 ± 0.05） was significantly higher than in control group （0.26 ± 0.03,P ＜ 0.05）,but lower than in sham group （0.68-± 0.08,P ＜ 0.05）.The LBP group had more myelinated axons of CNs （106.8 ± 17.9） than the control group （64.9 ± 13.9,P ＜ 0.05）,but fewer than the sham group （180.9 ± 15.3,P ＜ 0.05）.The LBP group had more NOS diaphorase positive nerve （164.2 ± 12.2） than the control group （85.6 ±14.7,P＜0.05）,but fewer than the sham group （263.3 ± 15.0,P＜0.05）.Conclusion The application of LBP after CN crush injury promotes nerve regeneration and erectile function recovery.

关 键 词：枸杞多糖 勃起功能障碍 海绵体神经 

分 类 号：R285.5[医药卫生—中药学]