胃转流术对2型糖尿病大鼠肝组织胰岛素受体底物-2、葡萄糖转运体-2表达的影响  

Expression of hepatic insulin receptor substrate-2 and glucose transporter-2 in rats with type 2 diabetes after gastric bypass operation

作　　者：张蓬波[1] 陈守坤[1] 张秀忠[1] 章红[1] 吕墩涛[1] 庄步强 温雨晴[1] 丁伟超[1] 任泽强[1] 

机构地区：[1]徐州医学院附属医院普外科,江苏221002

出　　处：《中华实验外科杂志》2013年第12期2587-2589,共3页Chinese Journal of Experimental Surgery

基　　金：徐州市科技计划资助项目（2012XM12B028）

摘　　要：目的 观察胃转流术（GBP）对2型糖尿病（T2DM）大鼠肝组织胰岛素受体底物-2（IRS-2）、葡萄糖转运体-2（GLUT-2）表达的影响,探讨GBP改善胰岛素抵抗的机制.方法 健康雄性SD大鼠随机分为糖尿病手术组（DO组）、糖尿病对照组（DC组）、正常手术组（NO组）、正常对照组（NC组）.术前及术后第1、2、4、8周分别测各组空腹血糖（FPG）、空腹胰岛素（FINS）水平,计算各组术前与术后第8周胰岛素敏感指数（QUICKI）.应用Western blot及逆转录-聚合酶链反应（RT-PCR）技术检测各组术后8周肝脏组织IRS-2、GLUT-2蛋白及mRNA的表达.结果 术后8周,DO组FPG由术前的（20.21±2.14） mmol/L下降至（8.50±2.19） mmol/L（P＜0.05）;DO组QUICKI由术前的0.43±0.02升至0.55±0.05（P ＜0.05）;DO组与DC组比较,IRS-2、GLUT-2的蛋白表达分别增加43％和40％ （P ＜0.05）,IRS-2、GLUT-2的mRNA表达分别增加28％和25％ （P＜0.05）.结论 GBP可上调T2DM大鼠肝脏组织胰岛素信号传导通路中IRS-2、GLUT-2的表达水平,提高胰岛素敏感性.Objective To observe the expression of insulin receptor substrate-2 （IRS-2） and glucose transporter-2 （GLUT-2） in rats with type 2 diabetes after gastric bypass operation （GBP）,and explore the possible mechanism of GBP improving insulin resistance.Methods Healthy male SD rats were randomly divided into type 2 diabetes-operation group （DO group） and type 2 diabetes-control group （DC group） ; normal-operation group （NO group） and normal-control group （NC group）.Plasma fasting glucose and insulin levels were measured respectively before and at 1st,2nd,4th and 8th week after operation.Quantitative insulin sensitivity check index （QUICKI） was measured respectively before and at 8th week after operation.The expression of hepatic IRS-2 and GLUT-2 protein and mRNa was detected by using Western blotting and reverse transcriptase-polymerase chain reaction （RT-PCR） respectively at 8th week postoperation.Results The fasting blood glucose levels in DO group were decreased from the preoperative （20.21 ±2.14） mmol/L to （8.50 ±2.19） mmol/L （P ＜0.05） at 8th week after GBP.The QUICKI in DO group was increased dramaticly from preoperative 0.43 ± 0.02 to 0.55 ± 0.05 at 8th week postoperation （P ＜ 0.05）.As compared with DC group,the expression of IRS-2 and GLUT-2 protein and that of IRS-2 and GLUT-2 mRNA at 8th week after operation in DO group were increased by 43％ and 40％ （P ＜0.05）,and 28％ and 25％ （P ＜ 0.05） respectively.Conclusion The expression of hepatic IRS-2 and GLUT-2 in rats with type 2 diabetes after GBP was up-regulated,and meanwhile the insulin sensibility was improved.

关 键 词：胃转流术 2型糖尿病 胰岛素受体底物-2 葡萄糖转运体-2 

分 类 号：R656.6[医药卫生—外科学]