大黄素对胆管癌QBC939细胞凋亡及丝裂原活化蛋白激酶信号转导途径表达的影响  

Inhibitory effects of Emodin on cholangiocarcinoma QBC939 cells proliferation and mitogen-activated protein kinase signal pathway in vitro

作　　者：王家兴[1] 周俊峰[1] 张琳[1] 潘敦[1] 黄发昆[1] 石铮[2] 何庆良[1] 

机构地区：[1]福建医科大学附属第一医院胃肠外科1区,福州350005 [2]福建医科大学附属第一医院肝胆胰外科,福州350005

出　　处：《中华实验外科杂志》2013年第12期2590-2592,共3页Chinese Journal of Experimental Surgery

基　　金：福建省科技厅自然科学基金资助项目（2011J01165）;福建省教育厅基金资助项目（JA11119）

摘　　要：目的 观察大黄素在体外对胆管癌QBC939细胞增殖的抑制作用及信号转导通路丝裂原活化蛋白激酶（MAPK）的影响.方法 用大黄素作为干预因素,剂量效应组分别用不同浓度大黄素的培养液与QBC939细胞共培养;检测细胞增殖、凋亡,逆转录-聚合酶链反应（RT-PCR）检测细胞中c-myc mRNA表达,Western blot检测细胞中c-myc、MAPK、p-MAPK蛋白质的表达.结果 大黄素抑制QBC939细胞增殖,0、20、40、80 μmol/L大黄素对QBC939细胞增殖抑制率分别为（4.73±0.88）％、（21.27±4.04）％、（38.68±4.57）％、（62.45±6.12）％ （P ＜0.05）,c-myc、p-MAPK蛋白明显下降,MAPK蛋白表达无变化.结论 大黄素可能通过抑制MAPK信号转导途径抑制QBC939细胞增殖.Objective To investigate the effects of Emodin in growing inhibition and down-regulating mitogen-activated protein kinase （MAPK） in vitro.Methods QBC939 cells were cultured with Emodin for different concentrations of Emodin.The inhibition of cell proliferation was detected by methyl thiazol tetrazolium （MTT）.The expressions of c-myc mRNA and protein were detected by reverse transcriptasepolymerase chain reaction （RT-PCR） and Western blotting,MAPK,p-MAPK was detected by Western blotting.Results Emodin inhibited cell proliferation of QBC939 cell.apoptosis rate of QBC939 cells treated with 0,20,40,80 μmol/L Emodin for48 h was （4.73 ±0.88）％,（21.27 ±4.04）％,（38.68 ±4.57） ％,（62.45 ± 6.12） ％ （P ＜ 0.05） Emodin could down-regulating c-myc,p-MAPK level （P ＜0.05）.but MAPK expression level had no significant change.Conclusion Emodin can inhibite cell proliferation of human cholangiocarcinoma cell QBC939.The mechanism is associated with down-regulating MAPK.

关 键 词：胆管癌细胞株QBC939 大黄素 丝裂原活化蛋白激酶信号通路 

分 类 号：R285.5[医药卫生—中药学] R735.8[医药卫生—中医学]