微小RNA-133a在心肌缺血再灌注损伤后心室重塑过程中的表达  

MicroRNA-133a expression in the ventricular remodeling process after myocardial ischemia-reperfusion injury

作　　者：薛才广[1] 梁德刚[1] 田轶魁[1] 钱兆洋[1] 杜鑫[2] 魏民新[1] 

机构地区：[1]天津医科大学总医院心血管外科,300052 [2]天津医科大学总医院心内科,300052

出　　处：《中华实验外科杂志》2013年第12期2640-2643,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81041042）;天津市应用基础及前沿技术研究计划项目（10JCZDJC19200）

摘　　要：目的 观察心肌缺血再灌注损伤后心室重塑的动态过程以及微小RNA（miRNA）-133a在此过程中的表达.方法 建立活体大鼠心肌缺血再灌注损伤模型.将实验动物随机分为假手术组（Sham组）、缺血再灌注损伤组（I/R组）.选取术后1、3、7、15、30d 5个观察点,将每组分为5个亚组,监测心脏功能,检测心室重塑指标以及心肌组织内miRNA-133a的表达.结果 I/R组：随再灌注时间延长心功能逐渐下降,心肌内Ⅰ型胶原蛋白表达明显上调,心肌细胞凋亡率明显升高（P＜0.05）,缺血区间质纤维化加重,心肌组织内miRNA-133a表达下调（P＜0.05）;其中第3天,细胞凋亡率最高,为（54.6±7.3）％,纤维化开始明显[L/R组比Sham组：（5.60±2.30）％比（1.64±0.41）％,P＜0.05],心肌组织内miRNA-133a表达量最低（0.57±0.22）.结论 心肌缺血再灌注损伤后发生纤维化重塑,再灌注后3d是心室重塑形成的重要时期;miRNA-133a在心肌缺血再灌注后心室重塑的动态过程中表达明显下调,可能参与调节此过程.Objective To observe the dynamic process of ventricular remodeling after myocardial ischemia-reperfusion injury,and to explore the expression of microRNA （miRNA）-133a in the dynamic process of ventricular remodeling.Methods The model of myocardial ischemia-reperfusion injury in rats was constructed.Healthy adult male wistar rats were randomly divided into two groups：（1） Sham group ; （2） I/R group.In postoperative 1,3,7,15,and 30d,cardiac function was analyzed,indicators of ventricular remodeling and miRNA-133a levels were detected.Results In L/R group：with reperfusion time extension,cardiac function was gradually declined.The expression of type I collagen were significantly higher.Apoptosis were significantly increased （P ＜ 0.05）.Ischemia area myocardial fibrosis was gradually worsened.miRNA-133a expression levels were markedly lowered （P ＜ 0.05）.On the 3 d after reperfusion,apoptosis rate was highest （54.6 ±-7.3） ％,Fibrosis became obvious [I/R VS Sham：（5.60 ± 2.30） ％ vs.（1.64 ± 0.41） ％,P ＜ 0.05],and miRNA-133a was the lowest （0.57 ± 0.22）.Conclusion Fibrosis remodeling occurred after myocardial ischemia-reperfusion.An important period of ventricular remodeling was from 3d after reperfusion.The miRNA-133a expression was significantly lowered in the process of ventricular remodeling after myocardial ischemia-reperfusion injury.miRNA-133a may be involved in the regulation of myocardial ischemia and reperfusion ventricular remodeling process.

关 键 词：微小RNA-133a 心肌缺血再灌注损伤 心室重塑 细胞凋亡 

分 类 号：R541[医药卫生—心血管疾病]