脾气虚证2型糖尿病大鼠模型的复制  

Replication of Deficiency Syndrome T2DM Rat Model

作　　者：杨宇峰[1] 王莉[1] 石岩[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032

出　　处：《辽宁中医杂志》2013年第12期2590-2592,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家科技部"十一五"国家科技支撑计划(2007BAI20B103)

摘　　要：目的：观察通过劳倦加饮食不节（高糖高脂膳食）复合腹腔注射链脲佐菌素造模方法能否成功复制出脾气虚证2型糖尿病动物模型。方法：健康雄性Wistar大鼠60只，体重（220±20）g，按体重随机分成二组：空白对照组（n=20）和模型组（n=40）。模型组单日喂食甘蓝15～20g/只，自由饮水，游泳至耐力极限；双日高糖高脂膳食持续喂养，共计12周，第4周后给予链脲佐菌素腹腔注射。结果：模型组大鼠造模开始后，出现了游泳耐力逐日下降，食少懒动，大便溏薄，精神萎靡不振，体毛光亮度减退，从第6周后体重就开始显著增加，链脲佐菌素腹腔注射2周后空腹血糖、餐后2h血糖增高，甘油三酯和总胆固醇分别从第4周后表现出异常，造模结束后模型组出现高血糖症、高胰岛素血症、胰岛素抵抗，符合本研究拟建立的脾气虚证、2型糖尿病的评估标准。结论：通过劳倦加饮食不节（高糖高脂膳食）造模方法可成功复制出脾气虚证2型糖尿病动物模型。Objective ： In this study, we observed that whether the modeling approach by fatigue plus improper diet （ high sugar high fat diet） and intraperitoneal injection of streptozotocincan can copy deficiency syndrome T2DM rat model. Methods：60 healthy male Wistar rats,weighing （220 ±20） g,were randomly divided into two groups after adaptive feeding days：blank control group （ n = 20） and model group （ n = 40）. The model group were fed cabbage 15x 20 g/rat in a single day and allowed to drink water freely, swimming to the endurance limit. The model group were fed high sugar, high fat diet feeding in a double day, a total of 12 weeks. We looked forward to copy the model of deficiency syndrome T2DM. Results ： Rats model showed swimming endurance was decreased, eating less, being lazy, loose and thin stools, spiritual malaise, and the body degree was diminished. The weight be-gan to increase significantly after 6 weeks. Fasting blood glucose was increased after the intraperitoneal injection of streptozotocin. Triglycerides and high density lipoprotein were abnormal after 4 weeks. Rats showed hyperinsulinemia, insulin resistance lining with spleen deficiency syndrome and T2DM evaluation criteria. Conclusion：The modeling approach by fatigue plus improper diet （high sugar high fat diet） and intraperitoneal injection of streptozotocincan can copy deficiency syndrome T2DM rat model.

关 键 词：脾气虚 2型糖尿病 动物模型 

分 类 号：R332[医药卫生—人体生理学]