四逆汤抗实验性动脉粥样硬化的作用及其机制  

Effect of Anti-Atherosclerosis of Sini Decoction and its Mechanism

作　　者：黄河清[1] 吴伟康[2] 程超[3] 

机构地区：[1]广东省第二中医院内科,广东省广州市510095 [2]中山医科大学中西医结合研究所 [3]中山医科大学附属第三医院

出　　处：《中国动脉硬化杂志》2000年第4期302-304,共3页Chinese Journal of Arteriosclerosis

基　　金：中国博士后科学基金资助 !(项目编号 199917)

摘　　要：为探讨四逆汤抗实验性动脉粥样硬化的作用及其可能机制 ,运用图像分析法测定主动脉内膜脂质斑块面积百分比 ,脂代谢指标分别运用酶动力学法、一步法、公式计算法、免疫透射比浊法等方法测定 ,血清一氧化氮及血浆内皮素分别用酶法及放射免疫法检测 ,血浆超氧化物歧化酶与丙二醛分别运用微量快速测定法、改良的八木国夫法测定。结果发现 ,四逆汤可明显缩小主动脉内膜脂质斑块面积 ,降低血清总胆固醇、甘油三酯、低密度脂蛋白胆固醇、载脂蛋白B及血浆内皮素和丙二醛浓度 ,提高血清一氧化氮水平、载脂蛋白A含量与血浆超氧化物歧化酶活性 ,四逆汤高剂量组效果为佳。结果提示 ,四逆汤具有较好的抗实验性动脉粥样硬化作用 ,其作用机制与调节脂代谢、抗脂质过氧化损伤及保护血管内皮细胞功能等有关。Aim To observe the effect of Sini decoction on plaque area (PA), nitric oxide (NO), endothelin (ET), superoxide dismutase (SOD), malondiadehyde (MDA) in experimental atherosclerosis rabbits and study the possible mechanism. Methods Detect PA of aortic endothelium with image analysis method. Lipid metabolism with enzyme dynamics method , one-step method , formula method and immunization photoextinction in dividually. Serum NO and plasma ET with enzyme method and radioimmunoassay method in dividually. Plasma SOD and MDA with microcontent fast detecting method and modified Ba MuGuoFu method in dividually. Results Sini decoction can diminish lipid plaque area of aortic endothelium, reduce total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDLC), apolipoprotein B (apo B) and plasma ET, MDA, improve serum NO, apolipoprotein A (apo A) and plasma SOD content . Sini-high-dose group has the best effect in this experiment ,presenting with dose-effect dependent relationship. Conclusions Sini decoction has good effect against atherosclerosis, the possible mechanism maybe related to regulation of lipid metabolism and clearance of free radical oxygen and protection of vascular endothelium.

关 键 词：四逆汤 中药 动脉粥样硬化 一氧化氮 

分 类 号：R259.435[医药卫生—中西医结合] R543.5[医药卫生—中医内科学]