氧化型脂蛋白(a)对鼠腹腔巨噬细胞增殖的影响  

The Effect of Oxidized Lipoprotein(a) on the Growth of Murine Peritoneal Macrophages

作　　者：张春妮[1] 庄一义[1] 吉维民[2] 刘小转[1] 强红娟[1] 

机构地区：[1]南京军区南京总医院全军医学检验中心,南京210002 [2]宝应县人民医院检验科

出　　处：《中国动脉硬化杂志》2000年第4期305-307,共3页Chinese Journal of Arteriosclerosis

基　　金：军队医药卫生科研基金资助(项目编号98H005)

摘　　要：为探讨氧化型脂蛋白 (a)对巨噬细胞增殖的影响 ,采用细胞计数和MTT掺入观察比较天然型脂蛋白(a)、氧化型脂蛋白 (a)和丙二醛修饰脂蛋白 (a)对培养的鼠腹腔巨噬细胞增殖的影响。结果发现 ,氧化型脂蛋白 (a)在蛋白浓度为 2 .5～ 40mg/L时刺激巨噬细胞增殖 ,蛋白浓度为 40mg/L时巨噬细胞数为对照组的 1.5倍 (P <0 .0 5 )。而天然型脂蛋白 (a)和丙二醛修饰脂蛋白 (a)对巨噬细胞增殖无影响。结果提示 ,氧化型脂蛋白 (a)可能通过促进巨噬细胞增殖加速动脉粥样硬化的发展。Aim To examine the effect of oxidized lipoprotein(a) on the proliferation of macrophages. Methods Murine peritoneal macrophages were cultured in vitro with 2.5～40 mg/L native lipoprotein(a), oxidized lipoprotein(a) or MDA-modified lipoprotein(a), and cells proliferation was evaluated by cell counting and MTT assay. Results When macrophages were incubated with 40 mg/L oxidized lipoprotein(a), cell numbers were increased about 1.5-fold compared with control group, while the growth of these cells was not stimulated by native lipoprotein(a) or MDA-modified lipoprotein(a). Conclusion Oxidized lipoprotein(a) might enhance the progression of atherosclerosis by promoting macrophages proliferation.

关 键 词：脂蛋白 巨噬细胞 腹腔 增殖 动脉粥样硬化 

分 类 号：R543.502[医药卫生—心血管疾病]