白及种子无菌繁殖微型种茎初探  

Study on the Sterile Propagation of Mini Seed-Stem Bletilla Striata Seed

作　　者：谢玲玲[1,2] 赵青华[2] 

机构地区：[1]建始县颐泰生物科技有限公司,湖北建始445300 [2]湖北省恩施州农业科学院,湖北恩施445000

出　　处：《湖北农业科学》2013年第19期4708-4709,共2页Hubei Agricultural Sciences

摘　　要：对白及[Bletilla striata(Thunb.ex A.Murray)Rchb.f.]种子无菌繁殖微型种茎进行了初步研究。结果表明,白及种子在KC+200 g/L马铃薯萌发培养基中发芽率最高,达到100%;在1/2 MS+0.2 mg/L NAA生根培养基中,白及各项指标最高,其中生根率为97%,最长根长为2.5 cm;在MS种茎培养基中白及的生长状态最好,平均每株产苗2.8个,最大叶长为3.8 cm,根数为6条/株。The experiment was carried out to investigate the sterile propagation of mini seed-stem by Bletilla striata seed on different media.The results showed that the seed germination rate of B.striata was highest with 100％ in KC ＋200 g/L potato germination medium; the rooting rate was 97％ in 1/2 MS＋0.2 mg/L NAA rooting medium,with the highest indicators and the longest root 2.5 cm; the growth was best in MS stem medium,with 2.8 seedlings per plant,the maximum leaf length 3.8 cm,and six roots per plant.

关 键 词：白及[Bletilla striata (Thunb EX A Murray)Rchb f ] 种子 繁殖 微型种茎 

分 类 号：S567.23[农业科学—中草药栽培] Q945.5[农业科学—作物学]