稻瘟菌激活蛋白基因的克隆及表达  

Cloning and Expression of Activitor Protein Gene of Magnaporthe grisea

作　　者：孙柏欣[1] 王疏[1] 邱德文[2] 

机构地区：[1]辽宁省农业科学院植物保护研究所,沈阳110161 [2]中国农业科学院植物保护研究所,北京100081

出　　处：《湖北农业科学》2013年第19期4807-4808,4811,共3页Hubei Agricultural Sciences

摘　　要：以提取的稻瘟菌(Magnaporthe grisea)基因组DNA为模板,PCR扩增出激活蛋白目的基因DW,构建融合表达载体pGEX-KG-DW并进行IPTG诱导表达。SDS-PAGE电泳分析结果表明,pGEX-KG-DW载体添加IPTG后能在BL21中表达,在68 ku处存在一条特异性带。Magnaporthe grisea genomic DNA as template,the activitor protein gene DW was cloned by PCR,and then the procaryotic expression vector pGEX-KG-DW was constructed and induced by IPTG.The SDS-PAGE results showed that the pGEX-KG-DW vector,which had the purpose protein induced by IPTG,was expressed in BL21,and there was a distinctive band at 68 ku.

关 键 词：稻瘟菌(Magnqporthe grisea) 激活蛋白 克隆 表达 

分 类 号：Q786[生物学—分子生物学]