几种因素对铁皮石斛原球茎增殖生长的影响  

Effect of several factors on growth and proliferation of Dendrobium candidum PLB during solid culture

作　　者：姚睿[1] 朴炫春[1] 邵春绘[1] 于丹[1] 廉美兰[1] 

机构地区：[1]延边大学长白山生物资源与功能分子教育部重点实验室,吉林延吉133002

出　　处：《广东农业科学》2013年第22期34-37,共4页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31160079)

摘　　要：为了优化铁皮石斛原球茎在固体培养茎中的增殖生长条件,为铁皮石斛原球茎大量繁殖提供理论依据,以铁皮石斛原球茎为试验材料,研究硝态氮和铵态氮浓度配比、PO43-浓度、糖种类、蛋白胨浓度和ABA浓度对铁皮石斛原球茎增殖生长的影响。结果表明,在固体培养基培养铁皮石斛原球茎的过程中,将硝态氮、铵态氮的浓度配比设置为25/5、将培养基中PO43-的浓度控制为2.1 mmol/L、以白糖作为碳源、添加3 g/L的蛋白胨、加入0.1 mg/L的ABA时有利于铁皮石斛原球茎的增殖生长。In order to optimize the conditions of proliferation of protocorm-like bodies （PLB） of Dendrobium candidum by solid culture, provide theoretical foundation for mass propagation of PLB, protocorms were used as experimental materials to study the effect of NO3-/NH4＋, PO43 concentration, sugar type, peptone and ABA concentration on the growth of PLB of Dendrobium candidum. The results showed that the proliferation and growth of PLB were best when the NO3-/NH4＋ was 25/5, the concentration of PO43- was 2.1 mmol/L , white sugar was used as carbon source, peptone concentration was 3 g/L and supplemented ABA was 0.1 mg/L during solid culture.

关 键 词：铁皮石斛 原球茎 增殖 

分 类 号：S567[农业科学—中草药栽培]