HLA—DRB等位基因与苏皖籍汉族人群甲真菌病的相关性初步研究  

Relationship between HLA-DRB alleles and onychomycosis in Chinese Han population in Jiangsu and Anhui provinces

作　　者：冯姣[1] 桑红[1] 周国华[3] 邹秉杰[3] 吴凡[2] 张敏 邓德权[1] 刘芳[1] 孔庆涛[1] 

机构地区：[1]南方医科大学南京临床医学院(南京军区南京总医院)皮肤科,南京210002 [2]南京大学医学院临床学院(南京军区南京总医院)皮肤科 [3]南京军区南京总医院药理科

出　　处：《中华皮肤科杂志》2013年第12期896-898,共3页Chinese Journal of Dermatology

摘　　要：目的探讨HLA—DRB等位基因与苏皖籍汉族人群甲真菌病的相关性。方法采用聚合酶链反应一序列特异性引物方法对50例红色毛癣菌甲真菌病患者、14例须毛癣菌甲真菌病患者和52例健康对照进行H1．A—DRB等位基因分型。应用SPSSforwindows13．0软件包，采用r检验比较甲真菌病患者组与对照组的HLA—DRB等位基因频率。结果红色毛癣菌甲真菌病患者HLA—DRB各等位基因频率与健康对照组相比差异均无统计学意义。须毛癣菌甲真菌病患者HLA—DRB1＊14等位基因频率为17．86％，较健康对照组（3．85％）升高（P〈0．01，OR=5．435，95％CI：1．353～21．835），HLA—DRB1＊15频率为0，较健康对照组（16．3％）下降（P〈0．05，OR=0．837，95％口：0．768～0．911），差异均有统计学意义。结论HLA—DRB等位基因可能与苏皖汉族人群甲红色毛癣菌感染无明显相关；HLA—DRB1＊14可能是甲须毛癣菌感染的易感基因，而HLA～DRB1＊15可能是甲须毛癣菌感染的捕抗基因；不同菌种感染所致的甲真菌病的遗传背景可能存在异质性。Objective To analyze the association of HLA-DRB alleles with onychomycosis in Chinease Han population in Jiangsu and Anhui provinces. Methods Polymerase chain reaction with sequence-specific primers （PCR-SSP） was conducted to analyze the type of HLA-DRB alleles in 50 patients with Trichophyton rubrum- induced onychomycosis, 14 patients with Trichophyton mentagrophytes-induced onychomycosis and 52 healthy human controls of Chinese Han nationality. Data were processed with the SPSS 13.0 software for Windows. The frequency of HLA-DRB alleles was compared between these groups using chi-square test. Results No significant differences were observed in the frequency of HLA-DRB alleles between the patients with Triehophyton rubrum- induced onychomycosis and healthy controls. The patients with Trichophyton mentagrophytes-induced onychomycosis showed significantly higher frequency of HLA-DRBI＊14 allele （17.86% vs. 3.85%, P 〈 0.01, OR -- 5.435, 95% CI： 1.353 - 21.835）, lower frequency of HLA-DRBI＊15 allele （0 vs. 16.3%, P 〈 0.05, OR = 0.837, 95% CI： 0.768 - 0.911 ）compared with the healthy controls. Conclusions In Chinese Han population in Jiangsu and Anhui provinces, HLA-DRB alleles appear to be unassociated with Triehophyton rubrum-induced onychomyeosis, whereas HLA-DRBI＊ 14 allele seems to be a suseeptible allele, and HLA-DRBl＊15 be a protective allele, for Trichophyton mentagrophytes-induced onychomycosis. Genetic backgrounds may be different for onychomycosis caused by different Trichophyton species in Chinese Han population.

关 键 词：B等位基因 甲真菌病 汉族人群 HLA DRB 相关性 红色毛癣菌感染 皖籍 

分 类 号：R756[医药卫生—皮肤病学与性病学]