Morphological and Molecular Identification of Two Strains of Dermatophytes  

作　　者：陈力 史国友 王梅梅 赵琳琳 黄园勇 陈霄磊 袁丽杰 熊亚南 司道文 朱丽华 

机构地区：[1]College of Clinical Medicine,Hebei United University [2]Jitang College,Hebei United University [3]Department of Pathogeny Biology,College of Basic Medicine,Hebei United University [4]Department of Pharmacy,Pharmaceutical College,Hebei United University [5]Department of Biotechnology,College of Life Sciences,Hebei United University [6]Department of Anatomy,College of Basic Medicine,Hebei United University

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2013年第6期917-922,共6页华中科技大学学报（医学英德文版）

基　　金：supported by the Doctoral Scientific Research Fund of Hebei United University(3907101162)

摘　　要：In this study, we used traditional morphological and molecular identification methods to preliminarily identify two strains of dermatophytes. The two strains were observed under the micro- scope. And then the dermatophytes were cultured on Sabouraud＇s dextrose agar （SDA）. The 18S rRNA regions of the two dermatophyte strains were amplified by polymerase chain reaction （PCR）, and the PCR products were sequenced and compared with GenBank data. BLAST tools and DNAMAN soft- ware were used to analyze the sequences. To further determine highly homologous sequences, a phy- logenetic tree was constructed using the Neighbor-Joining method. The two strains of dermatophytes were identified by traditional morphological identification as Epidermophyton floccosurn and Micro- sporum ferrugineum. The 18S rRNA sequence analyses showed high similarities to Cladosporium cladosporioides isolate Cll5LM-UFPR and Ascomycete sp. LB68A1A2. Epidermophyton and Cladosporium belong to dermatophyte, while Microsporum ferrugineum and Ascomycete belong to mi- crosporum. The two novel strains of dermatophytes were therefore identified as Cladosporium cladosporioides isolate C115LM-UFPR （JN650537, Cladosporium ） and Ascomycete sp. LB68A1A2 （AY770409, Ascomycete sp）.In this study, we used traditional morphological and molecular identification methods to preliminarily identify two strains of dermatophytes. The two strains were observed under the micro- scope. And then the dermatophytes were cultured on Sabouraud＇s dextrose agar （SDA）. The 18S rRNA regions of the two dermatophyte strains were amplified by polymerase chain reaction （PCR）, and the PCR products were sequenced and compared with GenBank data. BLAST tools and DNAMAN soft- ware were used to analyze the sequences. To further determine highly homologous sequences, a phy- logenetic tree was constructed using the Neighbor-Joining method. The two strains of dermatophytes were identified by traditional morphological identification as Epidermophyton floccosurn and Micro- sporum ferrugineum. The 18S rRNA sequence analyses showed high similarities to Cladosporium cladosporioides isolate Cll5LM-UFPR and Ascomycete sp. LB68A1A2. Epidermophyton and Cladosporium belong to dermatophyte, while Microsporum ferrugineum and Ascomycete belong to mi- crosporum. The two novel strains of dermatophytes were therefore identified as Cladosporium cladosporioides isolate C115LM-UFPR （JN650537, Cladosporium ） and Ascomycete sp. LB68A1A2 （AY770409, Ascomycete sp）.

关 键 词：DERMATOPHYTE IDENTIFICATION polymerase chain reaction gene sequencing 

分 类 号：R379[医药卫生—病原生物学]