低温等离子消融术建立兔椎间盘退变模型的实验研究  

Developing intervertebral disc degeneration at rabbit lumber spine by using low-temperature plasma radiofrequency ablation

作　　者：周葳[1] 徐义春[1] 骆众星[2] 王其友[1] 余冬青[2] 韦家冬[1] 余灏涛[1] 赵飞[1] 

机构地区：[1]中山大学附属第三医院脊柱外科,广州510630 [2]中山大学附属第三医院放射科,广州510630

出　　处：《中国临床解剖学杂志》2013年第6期696-701,共6页Chinese Journal of Clinical Anatomy

基　　金：广东省科技攻关项目(2007A060300005);广东省科技攻关项目(2009B030801095)

摘　　要：目的 探讨低温等离子消融术建立兔椎间盘退变模型的方法与可行性。方法 新西兰大白兔24只，随机分为实验组12只，对照组12只。两组均穿刺L3/4～L5/6椎间隙,实验组采用消融30s建模,对照组单纯以穿刺针穿刺建模。分别于术前及术后4、8、12周行CR、MRI检查及病理学检查。结果 实验组DR及MR检查至术后12周均可见退变逐步加重的征象，如椎间隙高度的丢失及T2信号逐步降低。对照组DR及MR检查4周后无明显的退变加重迹象。两组病理学检查均未见早期的髓核缺失，实验组12周可见髓核正常结构消失，椎间盘内结构紊乱。结论 低温等离子消融术比传统单纯穿刺更易及快速建立椎间盘退变的动物模型，采用此方法建立兔椎间盘退变模型是可靠可行的。Objective To develop a new intervertebral disc（IVD） degeneration animal model by plasma radiofrequency ablation. Methods 24 New Zealand white rabbits were equally divided into the experimental group and control group. L3/4～L5/6 IVDs were punctured and ablated for 30 seconds in the experimental group and t were just punctured in the control group. DR imaging studies, MR imaging studies and histological analysis were performed prior to surgery and 4, 8, 12 weeks after surgery to monitor progression of IVDs degeneration. Results The DR and MR examinations showed degeneration signs like lost of IVD height and T2 density. The control group showed no significant changes in DR and MR scan after 4 weeks. Both groups showed no lost of nucleus pulposus in the early time points and the experimental group showed lost of nucleus pulposus and derangement of the IVD structure. Conclusions The practicable and reproducible animal IVD degeneration model proposed in this paper is more stable than the previous puncture model.

关 键 词：低温等离子消融 椎间盘退变 动物模型 兔 

分 类 号：R681.5[医药卫生—骨科学]