急进高原后外周血单个核细胞内相关易感基因表达与急性高原反应分析  

The analysis between AMS and the correlated predisposing genes of ascentting of plateau in mononuclear cells of peripheral blood

作　　者：方盼盼[1,2] 宫璀璀[1,2] 仓宝成[1,2] 李正民[1,2] 李宗杰[1,2] 李文莉[2] 朱传杰[2] 王迎涛[2] 

机构地区：[1]郑州大学第三附属医院科研中心,河南郑州450052 [2]中国人民解放军第153中心医院中心实验室,河南郑州450042

出　　处：《基础医学与临床》2013年第12期1572-1575,共4页Basic and Clinical Medicine

基　　金：河南省基础与前沿技术研究计划(072300410440)

摘　　要：目的探讨低氧诱导因子1(HIF-1α)、P38 MAPK和血管内皮生长因子(VEGF)在急进高原后外周血单个核细胞的表达变化,以及其与急性高原反应(AMS)的关系。方法对某部队赴青海省玉树抗震的官兵分别于进入高原前后48 h采集外周血,提取单个核细胞用RT-PCR法检测P38 MAPK及HIF-1αmRNA表达,Western blot法检测HIF-1α及VEGF蛋白表达。按照《急性高原反应的诊断和处理原则》诊断AMS。结果与进入高原前相比,HIF-1αmRNA和蛋白表达水平及P38 MAPK mRNA表达水平均明显增高(P<0.001);VEGF蛋白水平也上调(P<0.01);HIF-1α及VEGF蛋白表达水平与AMS的发病程度高度相关,相关系数分别为0.875和0.675(P<0.001)。结论 HIF-1α、P38 MAPK和VEGF在急进高原48 h后表达水平均显著增加,HIF-1α和VEGF蛋白表达水平与AMS发病呈显著正相关。Objective To explore the change of expression of hypoxia inducing factor 1（HIF-1？ ）, P38MARK and vascular endothelial growth factor （VEGF ）after ascenting of plateau in vivo, and the correlation with the pathogenesy of AMS. Methods Collecting soldiers＇ venous blood before they asent of plateau and after they arrive in Yushu in Qinghai province 48 h later, extracting mononuclearcell the expression of mRNA of HIF-1？ and P38MARK were detected by RT-PCR and the expression of protein of HIF-1？ and VEGF was detected by Western blotting. The AMS is diagnosed according to《The Diagnose And Processing Principle Of AMS 》 Results After asentting of plateau the expression levels of mRNA of HIF-1？ , P38MARK and the protein of HIF-1？ and VEGF all increase significancely（p〈0.01）. At the same time, the change of expression levels of HIF-1？ and VEGF protein are highly corellated with AMS , the coefficient correlation is separately 0.806 and 0.675（p〈0.001）. Conclusion The expression level of HIF-1？, P38MARK and VEGF increase significantly after asentting of plateau, especially the changes of HIF-1？ protein and VEGF, it is highly correlated with the extent of AMS.

关 键 词：急性高原反应 低氧诱导因子1 P38MAPK 血管内皮生长因子 缺氧 

分 类 号：R594.3[医药卫生—内科学]