软鳍新光唇鱼(Neolissochilus benasi)的人工繁殖与胚胎发育  

Artificial propagation and embryonic development of Neolissochilus benasi

作　　者：潘晓赋[1] 刘倩[1] 王晓爱[1,2] 杨君兴[1] 陈小勇[1] 李再云[1] 李列 

机构地区：[1]中国科学院昆明动物研究所遗传资源与进化国家重点实验室,云南昆明650223 [2]中国科学院大学,北京1000493 [3]西畴县经济商务局,云南西畴663500

出　　处：《Zoological Research》2013年第6期617-625,共9页动物学研究（英文）

基　　金：云南大唐国际李仙江流域水电开发有限公司委托项目;国家电力云南阿墨江发电有限公司委托项目

摘　　要：2009—2011年，使用促黄体素释放激素类似物（LHRH-A2）及马来酸地欧酮（DOM）混合催产剂共催产软鳍新光唇鱼（Neolissochilus benasi）雌鱼60尾及雄鱼100尾，其中，雌鱼成功47尾（78.3%），雄鱼成功92尾（92.0%）。雌鱼产卵量为1 986~5 854 粒/尾，卵径为2.2~2.8 mm，平均核偏位率为73.2%。精子密度为（16.32±2.89）×109 个/ml，鲜精平均活力为（60.6±3.2）%，平均寿命为（70.2±5.3）s。胚胎发育过程需120 h，分为受精卵期、分裂期、囊胚期、原肠胚期、体节期和孵化期等6个阶段，平均孵化率为32.4%，45日龄仔鱼存活率为86.5%。胚胎发育过程中，畸形部位主要为口部、胸腔、脊索和眼部，且以脊索畸形较为常见，卵黄蘘吸收异常及双头亦为畸形表现形式。在上述数据的基础上，分析了软鳍新光唇鱼畸形的原因，并提出在亲鱼培育过程中应尽量提供适合的养殖条件，最大限度降低捕获诱发的压力，以提高繁殖效率。From 2009 to 2011, luteinizing hormone releasing hormone analogue （LHRH-A2） mixed with domperidon （DOM） was successfully applied during the artificial propagation of Neolissochilus benasi. Totally, 60 females and 100 males were injected with the hormone mixture, resulting in 47 （78.3%） females and 92 （92.0%） males being successfully spawned. A total of 1,986-5 854 eggs were spawned per female with an egg diameter varying between 2.2-2.8 mm, and an average nucleus deviation rate of 73.2%. Sperm density, vitality and life span were 16.32±2.89×109/mL, 60.6±3.2% and 70.2±5.3 s, respectively. On the whole, the embryonic development of N. benasi was similar to that of zebra fish-albeit relatively slower-lasting approximately 120 hours. The development itself can be divided into six discrete stages： zygote, cleavage, blastula, gastrula, segmentation and hatching. Results showed that the average hatching rate was 32.4%, with 86.5% of larvae surviving 45 days after hatching. During embryonic development, deformities commonly occurred on the mouth, chest, ocular region, especially in the spinal column. To try to attempt improving future breeding efforts, we provided a survey of the embryonic developmental difficulties of N. benasi using LHRH-A2 followed by several potential solutions, including providing suitable breeding conditions and minimizing capture stresses.

关 键 词：软鳍新光唇鱼 人工繁殖 胚胎发育 

分 类 号：S917.4[农业科学—水产科学]