心理应激干预大鼠颞下颌关节软骨中两种细胞因子的表达  

Psychological stress affects the expressions of two kinds of cytokines in rat temporomandibular joint cartilage

作　　者：黄旭[1] 肖朋[1] 王艳[1] 张红妤 刘海霞[1] 

机构地区：[1]新疆医科大学第二附属医院口腔科,新疆维吾尔自治区乌鲁木齐市830028

出　　处：《中国组织工程研究》2013年第46期8004-8011,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81360169)~~

摘　　要：背景:有动物建模方面的报道称心理应激可引起大鼠颞下颌关节疾病的发生,并有病理改变。目的:观察心理应激对大鼠颞下颌关节髁突软骨细胞血管内皮生长因子和转化生长因子β1表达的影响。方法:将48只成年Wistar雄性大鼠随机分为实验组和对照组,其中实验组36只,对照组12只。对实验组施加电击、制动、气候箱、食物诱惑等刺激,使实验组大鼠处于心理应激状态。将实验组分别于刺激3周(刺激3周组)、刺激6周(刺激6周组)和刺激6周后正常饲养6周(恢复组)时间点各处死12只;对照组12只同实验组最后一批一起处死,取颞下颌关节标本制作切片。结果与结论:恢复组血管内皮生长因子阳性率明显大于刺激3周组和对照组(P<0.05);刺激6周组转化生长因子β1阳性率明显大于对照组(P<0.05)。表明血管内皮生长因子与新生毛细血管修复组织有关,转化生长因子β1在心理应激过程中可能起到了促进骨吸收的作用。BACKGROUND： Animal modeling has reported that psychological stress can lead temporomandibular joint disease in rats, and there will be pathological changes. OBJECTIVE： To observe the effects of psychological stress on the expressions of vascular endothelial growth factor and transforming growth factor-~l in rat temporomandibular joint condylar chondrocytes METHODS： Forty-eight adult male Wistar rats were randomly divided into the experimental group and the contro group, 6 rats in the experimental group and 12 rats in the control group. Thirty-six Wister male adult rats in the experimental group were subjected to the following stimulating factors, such as electroshock, keeping stillness, climate change and food tempting in order to make them under psychological stress. Then, the experimental group rats were executed at 3 weeks after stimulation （3 weeks stimulation group）, 6 weeks after stimulation （6 weeks stimulation group） and 6 weeks normal feeding after 6 week stimulation （recovery group）, 12 rats were sacrificed at each time point. The 12 rats in the control group were executed at the same time as the last experimental group. The temporomandibular joint specimens were taken to produce slices. RESULTS AND CONCLUSION＂ The vascular endothelial growth factor-positive rate of recovery group was significantly larger than that of the 3 weeks stimulation group and the control group （P 〈 0.05）; transforming growth factor-~l positive rate in the 6 weeks stimulation group was significantly greater than that in the control group （P 〈 0.05）. It indicated that vascular endothelial growth factor was related with the new blood capillaries repairing tissue, and transforming growth factor-l1 might play a role that stimulates bone resorption in psychological stress.

关 键 词：组织构建 组织构建与生物活性因子 心理应激 颞下颌关节 颞下颌关节疾病 关节软骨 血管内皮生长因子 转化生长因子β1 免疫组化实验 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]