高压氧预处理损伤脊髓Bcl-2/Bax的表达  

Effect of hyperbaric oxygen preconditioning on expression of Bcl-2 and Bax after spinal cord injury

作　　者：张玉强[1] 李游[2] 曹阳[1] 董明岩[1] 吕刚[1] 

机构地区：[1]辽宁医学院附属第一医院骨科眼科,辽宁省锦州市121000 [2]辽宁医学院附属第一医院眼科,辽宁省锦州市121000

出　　处：《中国组织工程研究》2013年第46期8018-8023,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:研究表明高压氧治疗可通过减少脊髓损伤后神经细胞凋亡,促进脊髓神经功能的恢复,但其抑制凋亡的机制还不清楚。目的:探索高压氧预处理对脊髓损伤后Bcl-2和Bax表达的影响及意义。方法:将66只健康成年雄性SD大鼠随机分为假手术组6只、模型组和高压氧预处理组各30只。假手术组仅行椎板切除;模型组根据Allen’s打击原理制作脊髓损伤模型,不做任何治疗;高压氧预处理组在10 d内对大鼠进行5次,每次1 h的高压氧(0.25 MPa,纯氧)治疗后,再建立脊髓损伤模型。结果与结论:免疫组化检测显示假手术组大鼠脊髓组织中Bcl-2和Bax表达较少;模型组和高压氧预处理组大鼠脊髓组织中均可见不同程度的Bcl-2和Bax阳性表达,其中Bcl-2表达高峰出现在损伤后第7天,而Bax表达高峰出现在损伤后第3天;而经高压氧预处理的大鼠脊髓组织中Bcl-2表达增加(P<0.05),而Bax表达减少(P<0.05)。提示高压氧预处理能够增强Bcl-2的表达同时降低Bax的表达。BACKGROUND： Studies have shown that hyperbaric oxygen preconditioning can reduce neuronal apoptosis and improve neurologic dysfunction after spinal cord injury. However, the mechanism of inhibiting apoptosis is still unclear. OBJECTIVE： To study the effect and significance of hyperbaric oxygen preconditioning on expression of Bcl-2 and Bax protein after spinal cord injury in rats. METHODS： Sixty-six male adult Sprague-Dawley rats were divided into sham operation group, model group, treatment group at random. Animals in the sham group were six, and animals in the model and treatment groups were 30, respectively. Animals in the treatment group received hyperbaric oxygen preconditioning at 2.5 MPa （100% Oz）, once for 1 hour and totally five times within 10 days before establishment of spinal cord injury models. In the sham operation group, only laminectomy was done. In the model group, a spinal cord injury model was prepared using Allen＇s method. RESULTS AND CONCLUSION： The results of immunochemistry indicated that there were few Bcl-2 and Bax-positive cells in the sham operation group; a number of Bcl-2 and Bax-positive cells were visible in the model and treatment groups. Bcl-2 and Bax expressions reached the peak at day 7 and 3, respectively, after injury. After treatment with hyperbaric oxygen preconditioning, Bcl-2 positive cells decreased significantly, while Bax-positive cells decreased significantly （P 〈 0.05）. It indicates that the hyperbaric oxygen preconditioning can increase Bcl-2 expression but decrease Bax expression.

关 键 词：组织构建 组织构建与生物活性因子 高压氧预处理 高压氧 脊髓损伤 BCL-2 BAX 免疫组化 脊髓 

分 类 号：R318[医药卫生—生物医学工程]