高眼压动物模型的造模时间  

Time for establishing an animal model of acute high intraocular pressure

作　　者：张昆亚[1] 钱秀清[1] 刘志成[1] 

机构地区：[1]首都医科大学生物医学工程学院,北京市100069

出　　处：《中国组织工程研究》2013年第46期8043-8048,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(11102123;31070840);北京市教育委员会科技计划面上项目(KM 201110025009);北京市属市管高校人才强教计划资助项目(PHR201110506);北京市自然科学基金资助项目(3122010)~~

摘　　要：tbb U y0111背景:急性高眼压动物模型为研究青光眼提供了简单有效的途径,模型成功与否直接影响研究结果,目前对造模成功的判据多为观察性指标。目的:为前房穿刺所致的急性高眼压动物模型提供准确、可行的造模成功判定方法,观察特定高眼压下急性高眼压动物模型的成模时间。方法:利用连通器原理,将加压装置与内充2 mm水银球的玻璃管相连,通过读取水银球的流动,计算眼内液体流入量。采用前房灌注法,成功建立10只兔眼眼内压为4.9 kPa的急性高眼压动物模型,监测眼内液体流入量随造模时间的变化至液体不再流动30 min后结束实验。拟合眼内压液体流入量随造模时间的变化规律,计算急性高眼压动物模型造模成功的时间。结果与结论:兔眼眼球进水量(y)与加压时间(t)呈Logistic变化规律,即,其中参数U,b0和b1分别为957.44±313.22,0.023±0.008和0.980±0.003。当注水时间达到(282.93±42.68)min后,眼内压等于外界施加的高压,兔急性高眼压模型造模成功。BACKGROUND： An animal model of acute high intraocular pressure offers an easy and effective method to glaucoma research. Research results will be distorted if the animal model is not induced successfully. Nowadays, a successful animal model can only be identified by observational indexes. OBJECTIVE： To observe the induction time for an animal model of acute high intraocular pressure, and thereby to provide accurate and feasible method for identifying whether the animal model is induced successfully. METHODS： Acute high intraocular pressure animal models were induced by anterior chamber perfusion using communicating vessels-based device with a glass vessel filled with normal saline and 2-mm-long mercury. Liquid volume was perfused to rabbit eyes. A total of 10 animal models were prepared successfully at an intraocular pressure of 4.9 kPa. The experiment was complete till 30 minutes after the perfused liquid was not moved. At the certain intraocular pressure, the variation of fluid inflow over time was recorded, and the induction time for successful modeling was calculated. RESULTS AND CONCLUSION： The curve between fluid inflow into rabbit eyes （y） and induction time （t） could ] be fitted to logistic function, while U, b0and bl are 957.44＋313.22, 0.023＋0.008 and 0.980：1：0.003, respectively. The fluid inflow to the eye ball did not change when the induction time was over （282.93＋42.68） minutes, and the animal model of acute high intraocular pressure was established successfully.

关 键 词：组织构建 组织构建实验造模 兔眼 急性高眼压模型 连通器 Logistic函数 造模时间 眼球进水量 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]