辛伐他汀复合Bio-Oss修复兔下颌骨缺损  

Simvastatin compounded with Bio-Oss repairs rabbit mandibular defects

作　　者：王洋[1] 焦鑫[1] 梁恒燕[1] 葛振林[1] 

机构地区：[1]兰州大学口腔医(学)院正畸科,甘肃省兰州市730000

出　　处：《中国组织工程研究》2013年第48期8374-8380,共7页Chinese Journal of Tissue Engineering Research

基　　金：甘肃省自然科学研究基金资助项目(1208RJZA209)~~

摘　　要：背景:研究发现辛伐他汀具有促进新骨形成的作用,但其成骨机制及成骨效果目前仍然存在争议。目的:对比观察Bio-Oss/辛伐他汀复合材料与单纯Bio-Oss修复材料修复兔下颌骨骨缺损区的成骨效果。方法:12只新西兰大白兔下颌骨双侧制备缺损,随机将一侧采用辛伐他汀复合Bio-Oss修复缺损区;另一侧采用单纯Bio-Oss修复缺损区。两组均覆盖Bio-Gide胶原膜。植骨后4,8,12周分别处死各组兔子4只,通过大体观察,X射线及口腔锥形束CT影像学观察,组织学切片观察,定性定量对比分析植骨区牙槽骨形成情况。结果与结论:植骨后4,8,12周新骨形成逐渐增多,随着高阻射的Bio-Oss骨粉逐渐降解,在各时间点密度值测量结果辛伐他汀复合Bio-Oss组均显著低于单纯Bio-Oss组(P<0.05)。新生骨百分比测量结果辛伐他汀复合Bio-Oss均显著高于单纯Bio-Oss组(P<0.05)。提示辛伐他汀具有促进Bio-Oss骨粉吸收的效果,在骨缺损修复中具有促进新骨生成的作用。BACKGROUND： Studies have shown that simvastatin can promote bone formation, but there is still controversial on the osteogenic mechanism and osteogenic effect. OBJECTIVE： To explore the osteogenesis effect of the composite of simvastatin and Bio-Oss versus simple Bio-Oss material on the repair of rabbit mandibular defects. METHODS： Twelve New Zealand white rabbits were selected to establish alveolar bilateral mandibular defects models. The composite of simvastatin and Bio-Oss was implanted randomly in one side of defect region; Bio-Oss was simply implanted in the other side of defect region. Both sides were covered with Bio-Gide bilayer collagen membrane. Four rabbits in each group were sacrificed at 4, 8 and 12 weeks after implantation, and the general observation X-ray film, oral cone-beam CT imaging observation and histopathologic study and quantitatively were conducted to quantitatively and qualitatively comparative analyze the alveolar bone formation in the graft region. RESULTS AND CONCLUSION： At 4, 8 and 12 weeks after implantation, new bone formation was found and increased with time prolonging. With the gradual degeneration of high resistance fire Bio-Oss bone meal, the bone mineral density at different time points of the simvastatin composite Bio-Oss group was lower than that of the simple Bio-Oss group（P 0.05）. The percentage of bone formation in the simvastatin composite Bio-Oss group was significantly higher than that in the simple Bio-Oss group（P 0.05）. Simvastatin could accelerate Bio-Oss degradation and promote new bone formation in bone defects repairing.

关 键 词：骨关节植入物 骨关节损伤基础实验 辛伐他汀 骨缺损修复 引导骨再生技术 BIO-OSS BIO-GIDE 下颌骨 口腔锥形束CT 新骨生成 省级基金 

分 类 号：R318[医药卫生—生物医学工程]