Cell cycle-related genes p57kip2, Cdk5 and Spin in the pathogenesis of neural tube defects  

作　　者：Xinjun Li Zhong Yang Yi Zeng Hong Xu Hongli Li Yangyun Han Xiaodong Long Chao You 

机构地区：[1]Department of Neurosurgery,Deyang People's Hospital [2]Department of Neurobiology,the Third Military Medical University of Chinese PLA [3]Department of Neurosurgery,Sichuan Provincial People's Hospital [4]Department of Neurosurgery,West China Hospital of Sichuan University

出　　处：《Neural Regeneration Research》2013年第20期1863-1871,共9页中国神经再生研究（英文版）

基　　金：supported by the Science and Technology Key Program of Sichuan Provincial Health Ministry,No.080128

摘　　要：In the field of developmental neurobiology, accurate and ordered regulation of the cell cycle and apoptosis are crucial factors contributing to the normal formation of the neural tube. Preliminary studies identified several genes involved in the development of neural tube defects. In this study, we established a model of developmental neural tube defects by administration of retinoic acid to pregnant rats. Gene chip hybridization analysis showed that genes related to the cell cycle and apoptosis, signal transduction, transcription and translation regulation, energy and metabolism, heat shock, and matrix and cytoskeletal proteins were all involved in the formation of developmental neural tube defects. Among these, cell cycle-related genes were predominant. Retinoic acid treat-ment caused differential expression of three cell cycle-related genes p57kip2, Cdk5 and Spin, the expression levels of which were downregulated by retinoic acid and upregulated during normal neural tube formation. The results of this study indicate that cell cycle-related genes play an im-portant role in the formation of neural tube defects. P57kip2, Cdk5 and Spin may be critical genes in the pathogenesis of neural tube defects.In the field of developmental neurobiology, accurate and ordered regulation of the cell cycle and apoptosis are crucial factors contributing to the normal formation of the neural tube. Preliminary studies identified several genes involved in the development of neural tube defects. In this study, we established a model of developmental neural tube defects by administration of retinoic acid to pregnant rats. Gene chip hybridization analysis showed that genes related to the cell cycle and apoptosis, signal transduction, transcription and translation regulation, energy and metabolism, heat shock, and matrix and cytoskeletal proteins were all involved in the formation of developmental neural tube defects. Among these, cell cycle-related genes were predominant. Retinoic acid treat-ment caused differential expression of three cell cycle-related genes p57kip2, Cdk5 and Spin, the expression levels of which were downregulated by retinoic acid and upregulated during normal neural tube formation. The results of this study indicate that cell cycle-related genes play an im-portant role in the formation of neural tube defects. P57kip2, Cdk5 and Spin may be critical genes in the pathogenesis of neural tube defects.

关 键 词：neural tube defects NEURULATION gene chip cell cycle retinoic acid regulatory factor neural de-velopment regeneration neural regeneration 

分 类 号：R714.5[医药卫生—妇产科学]