实验动物雄性生殖系统比较组织学及自发病变研究  

Comparative histologic study of male reproduction system and pathological observation of spontaneous lesions in laboratory animals

作　　者：李涛[1] 王力[1] 徐文漭[1] 赵玺龙[1] 戴芳[1] 王媛媛[1] 杨丽琳[1] 宋蜀玲[1] 杨举伦[1] 

机构地区：[1]成都军区昆明总医院病理科,昆明650032

出　　处：《中国比较医学杂志》2013年第11期26-30,I0002,I0003,共7页Chinese Journal of Comparative Medicine

基　　金：云南省科技基础条件平台建设项目资助(2005PT05)

摘　　要：目的对实验动物雄性生殖系统进行比较组织学研究,找出其异同,为实验动物的基础研究提供正常组织学对照。方法选取实验动物质量国家检测标准检测合格的已经性成熟的昆明小鼠、SD大鼠、日本大耳白兔、比格犬、树鼩,无痛处死,行病理解剖、常规病理制片、HE染色、免疫组化染色,研究睾丸、附睾、前列腺、精囊腺、阴茎的组织学异同。结果 (1)日本大耳白兔曲细精管生精细胞层数最多,SD大鼠精子数量最多。(2)附睾被覆上皮存在差异性,日本大耳白兔附睾管上皮排列呈乳头状筛状结构,肌层最厚。(3)前列腺体积大小与种属有关,比格犬前列腺体积最大,腺体最多,发达。昆明小鼠、树鼩最小;平滑肌昆明小鼠最少,大鼠,树鼩最多。(4)SD大鼠精囊腺特别发达,体积大,呈囊状。上皮分支多且连接成网,腺腔内充满红色分泌物。比格犬无精囊腺。(5)昆明小鼠、SD大鼠及比格犬均有阴茎骨,昆明小鼠、SD大鼠阴茎骨旁有粘液腺包绕,可见骨髓腔,而比格犬阴茎骨旁无粘液腺。阴茎骨为成熟板层骨组织,无骨髓腔。比格犬阴茎内有更多厚壁血管及交感神经丛,并且阴茎海绵体内有较多脂肪细胞。昆明小鼠、SD大鼠有包皮腺,分别为浆液腺及混合腺(浆液+粘液腺)。包皮腺以昆明小鼠更为发达。(6)极少数实验动物存在炎症、溃疡、钙化、淋巴滤泡增生等自发病变。结论实验动物雄性生殖系统存在组织学的差异性,在进行动物实验和实验动物病理检测时应予考虑。Objective To study the comparetive histology of male reproductive system in five kinds of common laboratory animals, and to find out the similarities and differences among them to provide normal histologic controls for the basic research of the laboratory animal study. Methods Five kinds of animals, i.e. , Kunming mice ,SD rats, Japanese big ear rabbit, Beagle and tree shrews, which all reaching sexual maturity and their quality all meeting the national quality standards of the laboratory animal inspection, were selected to undergo pathological anatomy, pathological slices - making, conventional HE staining, immunohistochemical staining after painless death for the research of the histological similarities and differences on the testicles, epididymis, prostate, seminal vesicle gland and penis among them under microscope. Results （I） The maximum number of plies of spermatogenie ceils in seminiferous tubule is found in Japanese big ear rabbit, the maximum number of sperm counts is found in SD rat. （2） Differences are found in epididymis covering epithelium among the test animals, the epididymal epithelium of Japanese big ear rabbit is arranged in papillary and screen pipe structure, and its muscle layer is the thickest of all. （3） Different animal species varies in the size of prostate volume, Beagles have the biggest prostate volume and the most number of glands, while Kunming mice and Tree shrews have the minimum of that; The least smooth muscle amount is found in kunming mice, while The SD rats Rats and tree shrews have the most smooth muscle amount. （4） Seminal vesicle glands of the SD rats are obvious at its big volume and cystic, its epithelial cells have many branches and are connected into nets, its glandular cavity is filled with red secretion, while Beagle has no seminal vesicle gland at all. （5） Kunming mice, SD rats and beagle have penis bone, Kunming mice and SD rats have mucous glands wrapped around the penis bone with marrow cavity,while Beagle＇ s penis bone is make up of mature

关 键 词：实验动物 雄性生殖系统 比较组织学 自发病变 

分 类 号：R332[医药卫生—人体生理学]