大鼠50％减体积肝移植技术的改进  

The process optimization of 50% reduced-size liver transplantation in rats

作　　者：何勇[1] 姜骊[1] 鱼达[1] 谢海洋[1] 郑树森[1] 

机构地区：[1]浙江大学医学院附属第一医院浙江省器官移植重点研究实验室,杭州310003

出　　处：《中国比较医学杂志》2013年第11期61-63,I0008,共4页Chinese Journal of Comparative Medicine

基　　金：“浙江省重点科技创新团队”项目(2009R50038)

摘　　要：目的建立改良的大鼠50%减体积原位肝移植的模型。方法在经典的双袖套法大鼠肝移植模型的基础上,通过4次贯通穿刺、3次结扎、2次回折的肝脏缝扎方法,改善肝脏减体积切取的效果。结果所有50%减体积原位肝移植的大鼠均存活良好,肝功能迅速恢复正常,达到实验设计对动物模型的要求。结论采用本实验室规范的4贯通、3结扎、2回折的肝脏缝扎方法,可以建立稳定的50%大鼠同种异体减体积原位肝脏移植模型,值得推广应用。Objective To investigate the methodological improvement and process optimization of 50% reduced-size liver orthotopic transplantation model in rats. Methods Base on the classical double-bushing model, establish new process to improve the effect of liver resection, with the method of penetrated puncture 4 rounds, ligate 3 times and 2 folds back to graft the 50% reduced-size donor liver orthotopically. Results All rats remain good conditions after operation, liver function keep back to normal level quickly. Conclusions With the surgical novel graft method, a stable 50% reduced-size orthotopic liver transplantation model could be established,it meet the requirement of design of experiment of animal model, and is worth promoting.

关 键 词：大鼠 减体积肝移植 模型 动物 

分 类 号：R332[医药卫生—人体生理学]