聚乙二醇修饰Ⅰ型胶原后对其结构、性能的影响  

Effects on Structure and Performance of Type Ⅰ Collagen after Modified by Polyethylene Glycol

作　　者：薛媛[1,2] 刘新华[1,2] 琚海燕[1,2] 但年华[1,2] 但卫华[1,2] 

机构地区：[1]四川大学皮革化学与工程教育部重点实验室,四川成都610065 [2]四川大学生物医学工程技术研究中心,四川成都610065

出　　处：《中国皮革》2013年第23期10-14,22,共6页China Leather

基　　金：苏州市国际科技合作项目;项目编号:SH201108;四川大学青年教师科研启动基金项目编号:2011SCU11102;四川大学-德国司马公司创新奖学金资助

摘　　要：采用聚乙二醇(PEG)对Ⅰ型胶原进行了修饰,并采用红外、DSC、AFM、反应度、溶胀比以及吸水率,对修饰后I型胶原的结构、性能进行了表征。试验结果:红外光谱显示Ⅰ型胶原与PEG基本上为氢键结合;通过DSC试验发现随着PEG用量的增加,其变性温度先下降后上升,且PEG修饰I型胶原后的变性温度低于纯Ⅰ型胶原的变性温度;同时随着PEG用量的增加,其自由氨基数逐渐降低,反应度逐渐增加,吸水率逐渐降低;通过溶胀比试验发现Ⅰ型胶原经PEG修饰后其结构更加稳定,使得溶胀比降低;原子力显微镜的结果显示,经PEG修饰后,Ⅰ型胶原-PEG复合海绵上出现了很多的孔,且随着PEG用量的增加,孔分布得更加均匀密集。这说明了PEG与I型胶原的相容性较好,且PEG是一种优良的致孔剂。To investigate the structure and performance of type I collagen modified by polyethylene glycol （ PEG）, the FT - IR spectra, Differential Scanning Calorimheter （DSC）, Atomic Force Microscope （AFM）, degree of reaction , swelling rate and bib- ulous rate were adopted. The type I collagen and PEG are basically combined by hydrogen bond, which is demonstrated from the FT - IR spectra. The DSC experiment shows with the increase of PEG dosage, the denaturation temperature falls and then rises. And the denaturation temperature of type I collagen modified by polyethylene glycol is lower than that of pure type I collagen. At the same time, with the increase of PEG dosage, the number of free amino and bibulous rate gradually reduces, and the degree of reaction increases gradually. In the swelling rate experiment, we found that the structure of type I collagen is more stable after modified by PEG and the swelling rate decreases. Through the AFM experiment, we found that a lot of holes appear on the surface of type I collagen - PEG composite sponge. And the distribution of holes becomes uniformer and more intensive with the increase of PEG dosage. This study illustrates that the compatibility between type I collagen and PEG is good, and PEG is an excellent pore -forming agent.

关 键 词：I型胶原 聚乙二醇 修饰作用 结构 性能 

分 类 号：TS513[轻工技术与工程—皮革化学与工程]