桥本甲状腺炎和格雷夫斯病甲状腺组织中淋巴细胞亚群分布及临床意义  

Distribution of lymphocyte subsets in thyroid tissue in Graves' disease or Hashimoto's thyroiditis and its clinical significance

作　　者：李晓玲[1] 王存丰[2,3] 翟亚萍[2,4] 汪艳芳[2,5] 袁慧娟[2,5] 马跃华[2,5] 赵志刚[2,5] 

机构地区：[1]新乡医学院,2011级河南新乡453003 [2]郑州大学人民医院 [3]河南省人民医院超声科,郑州450003 [4]河南省人民医院血液病研究所,郑州450003 [5]河南省人民医院内分泌科,郑州450003

出　　处：《中华实用诊断与治疗杂志》2013年第12期1156-1158,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：国家自然科学基金项目(61151003)

摘　　要：目的研究桥本甲状腺炎(Hashimotos thyroiditis,HT)和格雷夫斯病(Gravesdisease,GD)患者甲状腺组织内淋巴细胞分布情况与特征。方法选择HT患者28例(HT组),GD患者15例(GD组),无功能的结节性甲状腺肿患者16例(对照组),在超声引导下行甲状腺细针穿刺获取甲状腺组织,采用流式细胞技术检测总T淋巴细胞(CD3+),辅助性T淋巴细胞(CD3+CD4+),细胞毒性T淋巴细胞(CD3+CD8+),调节性T淋巴细胞(CD4+CD25+CD127-),总B淋巴细胞(CD3-CD19+),总自然杀伤细胞(CD3-CD16+CD56+)的细胞数及CD4+/CD8+。结果与对照组比较,HT组CD3+总T淋巴细胞升高,CD4+CD25+CD127-调节性T细胞降低(P<0.05),GD组CD3-(CD16+/CD56+)自然杀伤细胞和CD4+CD25+CD127-调节性T细胞下降(P<0.05),CD3+CD4+的T淋巴细胞和CD4+/CD8+明显升高(P<0.05);HT组与GD组CD3+CD8+T淋巴细胞和CD3-CD19+B淋巴细胞及CD4+CD25+CD127-调节性T细胞比较差异均无统计学意义(P>0.05);HT组CD3+总T淋巴细胞及CD3-(CD16+/CD56+)自然杀伤细胞较GD组升高,CD3+CD4+T淋巴细胞及CD4+/CD8+较GD组下降(P<0.05)。结论 HT和GD患者甲状腺组织内淋巴细胞亚群分布发生偏移,提示淋巴细胞亚群分布的改变在自身免疫性甲状腺病发病起重要作用。Objective To study the distribution and characteristics of lymphocyte in thyroid tissue in the patients with Hashimoto＇s thyroiditis （HT） or Graves＇ disease （GD）. Methods A total of 28 patients with HT （HT group）, 15 patients with GD （GD group） and 16 patients with non-functioning thyroid nodules （control group） were obtained thyroid tissue by ultrasound-guided fine needle aspiration. Flow cytometry was used to detect CD3＋ T lymphocytes, CD3＋ CD4＋, CD3＋CD8＋ T cells, CD4＋ CD25＋CD127-Treg, CD3 CD19＋ B cells, CD3-CD16＋/CD56＋ narural killer ceils and CD4＋/CD8＋ . Results Compared with control group, CD3＋ T lymphocytes increased and CD4＋ CD25＋ CD127 Treg decreased in HT group （P〈0.05） , whereas CD3 （CD16＋/CD56＋ ） natural killer cells and CD4＋ CD25＋ CD127 Treg decreased and CD3＋ CD4＋ T cells and CD4＋/CD8＋ increased in GD group （P〈0.05）. There were no significant differences in CD3＋ CD8＋ T cells, CD3 CD19＋ B cells and CD4＋ CD25＋ CD127- Treg between HT and GD groups. CD3＋ T lymphocytes and CD3-（CD16＋/CD56＋） natural killer cells increased, and CD3＋ CD4＋ T and CD4＋/CD8＋ decreased in HT group in comparison with GD group （P〈0.05 ）. Conclusion The distribution of lymphocyte subpopulations deviates in thyroid tissue in patients with HT or GD, indicating that the distribution of lymphocyte subset is very important for the pathogenesis of autoimmune thyroid disease.

关 键 词：桥本甲状腺炎 格雷夫斯病 淋巴细胞亚群 甲状腺组织穿刺 

分 类 号：R581.1[医药卫生—内分泌]