猫眼外肌周围结缔组织结构分析  

Study on the connective tissue around extraocular muscle in cat

作　　者：孙春华[1] 赵堪兴[1] 林锦镛[1] 

机构地区：[1]天津市眼科医院天津医科大学眼科临床学院天津市眼科学与视觉科学重点实验室天津市眼科研究所, 300020

出　　处：《中华实验眼科杂志》2013年第12期1122-1124,共3页Chinese Journal Of Experimental Ophthalmology

基　　金：国家自然科学基金项目（30371520）

摘　　要：背景眼外肌周围的结缔组织成分对眼外肌的功能活动密切相关，猫的双眼位置与灵长类相似，但猫眼外肌的Pully结构与灵长类动物Pully结构和功能的异同尚不清楚，相关的研究结果对推测Pully结构与功能的关系有一定的帮助。目的观察猫的眼外肌周围结缔组织结构，探讨这一结构与眼球运动的功能关系。方法成年家猫5只，用盐酸氯胺酮注射行全身麻醉，将所有家猫眼眶分离取材并整体固定，每只动物任选一侧眼眶行大体解剖学观察，对侧眼眶行全眶冠状位8μm及5μm厚连续切片，相邻切片用Masson三色染色和Weigert染色法分析猫眼4条直肌周围结缔组织成分，并用α-平滑肌肌动蛋白（α—SMA）单克隆抗体进行免疫组织化学染色，鉴定眼眶平滑肌。结果Masson三色染色显示，猫眼外肌胶原纤维环较薄，与眶层肌纤维的联系疏松；胶原纤维环及连接带中平滑肌及弹性纤维仅以少量细胞的形式散在分布；内直肌周围胶原纤维环、内下侧连接带亦与其他纤维环或连接带无明显差异。结论猫眼外肌周围结缔组织较薄可能与猫双眼运动功能不甚发达有关。Background The extraocular muscles （EOMs） Pulley is considered as the functional origins of the reeti EOMs,it is determinants of ocular motility. Objective The structure of the connective tissue around EOMs in cat was studied,and the role of EOMs Pulley to ocular movement was investigated. Methods Five adult cats were involved. The gross anatomy of an orbit in each cat was observed. The other orbit was processed with paraffin imbedding and coronal serial sections. A murine monocolonal antibody to α-smooth muscle actin （α-SMA） was used to show smooth muscle, while Masson trichrome stain was used to show muscle and collagen, and Weigert stain to show elastin. Results An encircling ring of collagen circled EOM was thin and connected to the orbital layer of muscle fiber loosely. Less elastin fibers and little smooth muscle ceils were embedded in the collagen ring and connective band. Collagen ring around medial rectus and the connective band between medial rectus and inferior rectus was not more significantly developed than other bands. Conclusions The connective tissue around EOMs in cat may be related to its function of ocular movement, which is not developed.

关 键 词：眼外肌 结缔组织 PULLEY 眼球运动 猫 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]