罗哌卡因对大鼠骨髓间充质干细胞增殖和迁移能力的影响  

Effect of ropivacaine on proliferation and migration of rat bone marrow mesenchymal stem cells

作　　者：郭晓姝[1] 巩继平[2] 杨根庆[3] 常永丽[1] 高莉晶[1] 田小霞[4] 

机构地区：[1]长治医学院生理学教研室,山西长治046000 [2]新乡市中心医院麻醉科,河南新乡453000 [3]新乡医学院第三附属医院检验科,河南新乡453003 [4]长治医学院病理生理学教研室,山西长治046000

出　　处：《中南大学学报（医学版）》2013年第11期1152-1159,共8页Journal of Central South University ：Medical Science

基　　金：山西省高等学校科技研究开发项目(20091133)~~

摘　　要：目的:探讨罗哌卡因对大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)增殖能力和迁移能力的影响,为BMSCs的临床应用提供进一步的研究基础。方法:使用贴壁法分离扩增大鼠BMSCs,流式细胞仪检测细胞表面分子标志物;成脂、成骨和成肌诱导分化检测细胞的分化潜能;分别使用CCK-8和Brdu掺入方法检测不同浓度罗哌卡因处理对BMSCs增殖能力的影响;细胞划痕实验和Millicell小室迁移实验检测不同浓度罗哌卡因对大鼠BMSCs迁移能力的影响。结果:培养所得细胞具备BMSCs的表面标志物特点和多向分化潜能。50,100μmol/L罗哌卡因处理可显著降低BMSCs的增殖速度,降低BMSCs的Brdu阳性率,与对照组比较差异有统计学意义(P<0.05)。划痕实验和迁移实验表明罗哌卡因处理可引起BMSCs迁移能力的明显降低(P<0.05)。上述效应均对罗哌卡因的给药剂量呈现明显的剂量依赖关系,组间比较差异有统计学意义(P<0.05)。结论:罗哌卡因可显著降低大鼠BMSCs的增殖能力和细胞迁移能力,提示外科应用BMSCs时需注意罗哌卡因等长效局部麻醉剂对BMSCs造成的不利影响。Objective：To observe the inlfuence of ropivacaine on the proliferation and migration of rat bone marrow mesenchymal stem cells （BMSCs） and provide basis for the clinical application of BMSCs.Methods：Rat BMSCs were isolated and cultured by adherence method. Surface markers of BMSCs were examined by lfow cytometry. Multipotent differentiation of BMSCs was detected by induced adipogenesis, osteogenesis and muscular differentiation. Proliferation of BMSCs was examined by CCK-8 and Brdu incorporation atfer ropivacaine treatment at different concentrations. Migration of BMSCs was tested by cell scratch assay and Millicell experiment. Results：Cultured cells had representative appearance and surface markers of BMSC, and they had potential multiple differentiation. Ropivacaine treatment at 50 and 100μmol/L signiifcantly reduced the proliferation rate of BMSCs and Brdu incorporation rate. There was significant difference compared with the control group （P Conclusion：Ropivacaine can signiifcantly reduce the proliferation and migration of rat BMSCs, suggesting that the influence of local anesthetics on BMSCs has to be taken into account when BMSCs are used in clinical practice.

关 键 词：间充质干细胞 罗哌卡因 细胞增殖 细胞迁移 

分 类 号：R965[医药卫生—药理学]