无环鸟苷抗角膜病毒新途径的实验研究  

An experimental study of a new route for Acyclovir administration for anti keratitis therapeutic treatment

作　　者：王滨生[1] 滕雯[1] 韩晓梅 邢琳 江伟士 谷静芝 徐凯健 陆义成[3] 

机构地区：[1]哈尔滨医科大学第二临床医学院眼科教研室,哈尔滨150086 [2]哈尔滨医科大学,哈尔滨150086 [3]哈尔滨医科大学药学部

出　　处：《中华实验和临床病毒学杂志》2000年第4期379-381,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：黑龙江省科委九五攻关课题基金资助!(G95C2 449)

摘　　要：目的　研究用离心法制得的角膜胶原膜载释无环鸟苷的意义。方法　胶原膜载释药物与结膜下注射法按时间把 2 8只白兔分四组 ,每组 7只 ,左、右眼对照 ,对分组采集的房水用高效液相色谱分析法测定其中的药物浓度 ,做比较分析。结果　 0 .5h结膜下注射组房水中无环鸟苷显著高于胶原膜载药组 (t=4.0 5 ,P <0 .0 1) ;1h差异无统计学意义 (t=2 .0 74,P <0 .0 5 ) ,3和 5h胶原膜组房水中药物浓度均高于结膜下注射组 (t值分别是 4.76 1和 4.190 ;P <0 .0 1) ,并可维持较长时间。Objective To study the significance of a collagen shield made by centrifugal method that can deliver Acyclovir.Methods 28 white rabbits were divided into four groups for observing releasing of Acyclovir into aqueous at 0.5,1,3,5 hours after adding drugs in two different administrative ways,that were putting drug loaded collagen shields on one eye of the rabbit and subconjunctival injection of drug in another eye of the rabbit as control. The drug concentrations in aqueous of rabbit at different times using different administrative routes were measured by high performance liquid chromatography (HPLC). Results At 0.5 hr of drug administration, the Acyclovir concentrations in aqueous of subconjunctival injected rabbits were obviously higher than that in collagen shields loaded rabbits ( t = 4.050 , P < 0.01 ),but it became not so notable at the time of 1 hr.( t =2.074, P > 0.05 ). At the time of 3 hrs and 5 hrs, the drug concentrations in aqueous of collagen shield loaded eyes of the rabbits not only were higher than that of the subconjunctival injected rabbits ( t = 4.761 , t = 4.190 ,respectively, P < 0.01 ), but also could sustain rather a long time. Conclusion A drug delivering way using collagen shield made by centrifugal method can replace the subconjunctival injection.

关 键 词：局部投药 胶原 人工膜 阿昔洛韦 抗病药物 

分 类 号：R978.7[医药卫生—药品]