共表达PRRSV GP5和N蛋白重组杆状病毒的构建与鉴定  

Construction of Recombinant Baculovirus for Co-expressing PRRSV GP5 and N Proteins

作　　者：杨雷 漆世华 温文生 李玉萍 王焕君 谢红玲 

机构地区：[1]武汉中博生物股份有限公司,武汉430070

出　　处：《中国兽药杂志》2013年第12期13-16,共4页Chinese Journal of Veterinary Drug

基　　金："十二五"农村领域国家科技计划课题"家畜病毒病基因工程疫苗创制"(2011AA10A208)

摘　　要：利用杆状病毒双表达系统构建高致病性猪繁殖与呼吸综合征病毒(HP-PRRSV)N蛋白和GP5蛋白的重组杆状病毒Bac-N-GP5,对重组病毒进行间接免疫荧光、Western blot分析以及动物免疫试验。结果表明:重组杆状病毒中表达了重组N和GP5蛋白,重组杆状病毒免疫小鼠后产生了抗PRRSV GP5蛋白和N蛋白的特异性抗体。本试验成功构建共表达PRRSV N蛋白和GP5蛋白的重组杆状病毒,为下一步应用该重组杆状病毒作为基因工程亚单位疫苗奠定基础。In order to combine the humoral immunity of GP5 protein and cellular immunity of N protein of highly pathogenic porcine reproductive and respiratory syndrome virus, identification by indirect immunofluorescence, western blot, results showed that recombinant baculoviruses Bac -N -GP5 co -expressed the N and GP5 proteins. After immunization in BALB/c mice with cell lysates treated by aluminium adjuvant, Bac -N -GP5 induced strong N and GP5 - specific ELISA antibodies. These results suggested that the recombinant baculoviruses can be a potential genetic engineering vaccine against PRRSV infections.

关 键 词：猪繁殖与呼吸综合征 共表达 N蛋白 GP5蛋白 

分 类 号：S852.65[农业科学—基础兽医学]