IL-12对日本血吸虫重组SjGST-Sj32蛋白保护性免疫效果的影响  

SCHISTOSOMA JAPONICUM:PROTECTIVE IMMUNITY ENHANCED BY IL-12 IN MICE IMMUNIZED WITH RECOMBINANT SjGST-Sj32

作　　者：蔡春[1] 易新元[1] 周金春[1] 张顺科[1] 舒新华[1] 曾宪芳[1] Larry McReynolds 

机构地区：[1]湖南医科大学寄生虫学教研室,湖南长沙410078 [2]New England Biolab

出　　处：《中国寄生虫病防治杂志》2000年第4期288-290,共3页Chinese Journal of Parasitic Disease Control

摘　　要：为进一步提高重组 Sj GST- Sj32 (r Sj GST- Sj32 )的免疫保护作用 ,用 IL- 12作为佐剂进行免疫增强效果的观察。在 0、2、6周用 r Sj GST- Sj32经皮下免疫小鼠 3次 ,攻击感染后 1、3、5、7、9d经腹腔注射佐剂 IL- 12 ,用减虫率及减卵率表示保护性免疫力。结果与 PBS对照组相比 ,r Sj GST- Sj32 /IL- 12组减虫率为 38.2 % (P<0 .0 1) ,每克肝卵数 (L EPG)减少率为 75 .1% (P<0 .0 1) ,每雌肝卵数 (L EPF)减少率为 72 .9% (P<0 .0 1) ;与单独注射 r Sj GST- Sj32组相比 ,r Sj GST- Sj32 /IL- 12组减虫率为 2 6 .8% (P<0 .0 5 ) ,L EPG减少率为 72 .5 % (P<0 .0 1) ,L EPF减少率为6 6 .2 % (P<0 .0 5 )。表明 IL- 12作为佐剂 ,可同时增强 r Sj GST- Sj32诱导小鼠产生的抗感染和抗生殖免疫保护力。In order to enhance protective immunity against Schistosoma japonicum , IL 12 was administered to mice as an adjuvant in conjunction with rSjGST Sj32. In vaccination group BALB/c mice were subcutaneously immunized with rSjGST Sj32 at week 0,2,4, IL 12 was injected on days 1 through 3,5,7,9 following challenge. Reduction in worm burden and egg load were calculated. The results showed that mice immunized with rSjGST Sj32 plus IL 12 had a 38.2%( P <0.01) reduction in worm burden, 75.1%( P <0.01) reduction in liver egg per gram(LEPG) and 72.9% ( P <0.01) reduction in liver egg per female(LEPF) compared with control group, mice immunized with rSjGST Sj32 plus IL 12 had a 26.8%( P <0.05) reduction in worm burden, 72.5%( P <0.01) reduction in LEPG and 66.2% ( P <0.05) reduction in LEPF compared with vaccination group of rSjGST Sj32. The results suggested that IL 12 could enhance the protective immunity induced by vaccination with rSjGST Sj32.

关 键 词：日本血吸虫 重组蛋白质 IL-12 免疫 

分 类 号：R383.24[医药卫生—医学寄生虫学]