模拟运动病Ca^(2+)内流机制探讨  

A study of the mechanism of Ca^(2+)inflow in simulated motion sickness of rat

作　　者：王琰[1] 徐根兴[1] 董文度[1] 袁驾南[1] 谢培增 巫立新 金淑仪[2] 姜正林[2] 李建成[2] 庄坚[2] 

机构地区：[1]江苏省南京中国人民解放军第二军医大学南京军医学院细胞生物学研究室,210099 [2]南通医学院航海医学研究所

出　　处：《中华航海医学杂志》2000年第4期197-200,共4页

摘　　要：目的　探讨模拟运动病的发病机制。方法　采用正负交变加速度旋转刺激制备大鼠运动病模型 ,用细胞化学、免疫组化、原位杂交及图像分析等技术对运动病大鼠大脑皮质、小脑皮质、脑干前庭区脑细胞的 Ca2 + 、c- fos m RNA、Fos蛋白、Na+ - K+ - ATP酶、细胞色素氧化酶、碱性磷酸酶等进行检测 ;对运动病大鼠血浆 TXB2 、6 - keto- PGF1α和心钠素等用放射免疫法测定。结果　旋转刺激后 ,大脑皮质、小脑皮质和脑干前庭区脑细胞中的 Ca2 +、c- fos m RNA、Fos蛋白含量及血浆 TXB2 、6 - keto- PGF1α和心钠素含量均有所上升 (P<0 .0 1或 P<0 .0 5 ) ;大脑皮质、小脑皮质和脑干前庭区脑细胞中的碱性磷酸酶的活性均显著增强 (P<0 .0 1) ;而 Na+ - K+ - ATP酶及细胞色素氧化酶活性则显著降低 (P<0 .0 1)。结论　大鼠脑细胞 Ca2 +内流和脑血流减少是诱发运动病的重要因素之一 ,用脑益嗪处理大鼠以阻滞 Ca2 +内流和增加脑血流后 ,可减轻运动病大鼠 c- fos m RNA、Fos蛋白、Na+ - K+ - ATP酶、细胞色素氧化酶、碱性磷酸酶、TXB2 、6 - keto-Objective To study the mechanism of motion sickness.Methods Rat models of motion sickness were induced by alternating rotational stimulation in an accelerator.The changes of Ca 2+ ,c fos mRNA,Fos protein,Na + K + ATPase, cytochrome oxydase(CCO)and alkaline phosphatase(AKP) in the cerebral cortex,cerebellar cortex and vestibular area of brain stem of rats with motion sickness were detected by the methods of cytochemistry,cytoimmunochemistry,in situ hybridization and image analysis.The amounts of plasma TXB 2,6 keto PGF 1α and cardionatrin were measured by radioimmunoassay.Results After rotational stimulation,significant increases of Ca 2+ ,c fos mRNA and Fos protein in the neurons of these brain areas and also TXB 2,6 keto PGF 1α and cardionatrin in the plasma were measured( P <0 05 or P <0 01).The AKP activity in these neurons rose markedly( P <0 01)whereas Na + K + ATPase and CCO activity significantly lowered( P <0 01).In the cinnarizine treated group,a tendency of change toward normal was noticed( P <0 05 or P <0 01),but little change of cardionatrin was detected( P >0 05).Conclusions It is suggested that Ca 2+ inflow into the neurons and the decrease of cerebral blood flow might be important factors in inducing motion sickness.Cinnarizine can be used to block the Ca 2+ inflow,increase the cerebral blood flow and diminish the changes of Ca 2+ ,c fos mRNA,Fos protein in the brain and also plasmic TXB 2,6 keto PGF 1α and the CCO and AKP enzyme activities.

关 键 词：运动病 前庭刺激 钙离子 C-FOS基因 脑益嗪 

分 类 号：R87[医药卫生—运动医学]